一种用于凋亡小体荧光标记的色氨酸-硼二吡咯烯环状肽。
A Trp-BODIPY cyclic peptide for fluorescence labelling of apoptotic bodies.
作者信息
Subiros-Funosas Ramon, Mendive-Tapia Lorena, Sot Jesus, Pound John D, Barth Nicole, Varela Yaiza, Goñi Felix M, Paterson Margaret, Gregory Christopher D, Albericio Fernando, Dransfield Ian, Lavilla Rodolfo, Vendrell Marc
机构信息
MRC/UoE Centre for Inflammation Research, The University of Edinburgh, 47 Little France Crescent, EH16 4TJ Edinburgh, UK.
Department of Inorganic and Organic Chemistry, University of Barcelona, Martí i Franquès 1-11, 08028 Barcelona, Spain.
出版信息
Chem Commun (Camb). 2017 Jan 10;53(5):945-948. doi: 10.1039/c6cc07879f.
The rational design and synthesis of a Trp-BODIPY cyclic peptide for the fluorescent labelling of apoptotic bodies is described. Affinity assays, confocal microscopy and flow cytometry analysis confirmed the binding of the peptide to negatively-charged phospholipids associated with apoptosis, and its applicability for the detection and characterisation of subcellular structures released by apoptotic cells.
本文描述了一种用于凋亡小体荧光标记的色氨酸-硼二吡咯（Trp-BODIPY）环肽的合理设计与合成。亲和力测定、共聚焦显微镜和流式细胞术分析证实了该肽与凋亡相关的带负电荷磷脂的结合，以及其在检测和表征凋亡细胞释放的亚细胞结构方面的适用性。
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