State Key Laboratory of Separation Membranes and Membrane Processes, School of Environmental and Chemical Engineering, Tianjin Polytechnic University, Tianjin 300387, PR China.
State Key Laboratory of Separation Membranes and Membrane Processes, School of Environmental and Chemical Engineering, Tianjin Polytechnic University, Tianjin 300387, PR China.
Anal Chim Acta. 2017 Feb 8;953:63-70. doi: 10.1016/j.aca.2016.11.049. Epub 2016 Nov 25.
In this paper, a simple and effective method was designed to synthesize hydrophobic carbon dots. Subsequently, amphiphilic fluorescent carbon dots (A-CDs) were synthesized by further surface modification. The result A-CDs show excellent optical properties with a quantum yield of 16.9%. It was interestingly found that morin (MR) and its fluorescent metal-ion complex (MR-Al) can successfully coordinate on the surface of A-CDs, the emission of A-CDs completely overlapped the absorption peak of MR-Al. Thus, the prepared A-CDs can be used as an effective fluorescent probe for Al based on a fluorescence resonance energy transfer process. The sensing platform can realize real-time detection of Al within 0.5 min. The fluorescence signals of the system were linearly correlated with the concentration of Al over a range of 8-20 μM, with a detection limit of 0.113 μM. The method was also successfully applied to image the distribution of Al in Human Umbilical Vein Endothelial Cells.
本文设计了一种简单有效的方法来合成疏水性碳点。随后，通过进一步的表面修饰合成了两亲性荧光碳点（A-CDs）。结果表明，A-CDs 具有优异的光学性能，量子产率为 16.9%。有趣的是，发现桑色素（MR）及其荧光金属离子配合物（MR-Al）可以成功地在 A-CDs 表面配位，A-CDs 的发射完全与 MR-Al 的吸收峰重叠。因此，所制备的 A-CDs 可以基于荧光共振能量转移过程用作 Al 的有效荧光探针。该传感平台可以在 0.5 分钟内实现 Al 的实时检测。该系统的荧光信号与 Al 的浓度在 8-20 μM 范围内呈线性相关，检测限为 0.113 μM。该方法还成功地用于在人脐静脉内皮细胞中成像 Al 的分布。
