Lungs for Living Research Centre, UCL Respiratory, Division of Medicine, and.
UCL Cancer Institute, University College London, London, United Kingdom.
Am J Respir Cell Mol Biol. 2021 Jun;64(6):657-668. doi: 10.1165/rcmb.2020-0440TR.
Advances in stem cell biology and the understanding of factors that determine lung stem cell self-renewal have enabled long-term culture of human lung cells derived from airway basal and alveolar type II cells. Improved capability to expand and study primary cells long term, including in clonal cultures that are recently derived from a single cell, will allow experiments that address fundamental questions about lung homeostasis and repair, as well as translational questions in asthma, chronic obstructive pulmonary disease, pulmonary fibrosis, and lung cancer research. Here, we provide a brief history of postnatal lung epithelial cell culture and describe recent methodological advances. We further discuss the applications of primary cultures in defining "normal" epithelium, in modeling lung disease, and in future cell therapies.
干细胞生物学的进步以及对决定肺干细胞自我更新的因素的理解，使人们能够长期培养源自气道基底和肺泡 II 型细胞的人肺细胞。改进的长期扩增和研究原代细胞的能力，包括最近从单个细胞衍生而来的克隆培养，将允许进行解决肺内稳态和修复基本问题以及哮喘、慢性阻塞性肺疾病、肺纤维化和肺癌研究中转化问题的实验。在这里，我们简要介绍了出生后肺上皮细胞培养的历史，并描述了最近的方法学进展。我们进一步讨论了原代培养在定义“正常”上皮、模拟肺疾病以及未来细胞治疗中的应用。
