Environment Research Institute, School of Environmental Science and Engineering, Shandong University, Ji'nan 250100, China.
Environment Research Institute, School of Environmental Science and Engineering, Shandong University, Ji'nan 250100, China; Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP), Fudan Tyndall Centre, Department of Environmental Science & Engineering, Fudan University, Shanghai 200433, China.
Sci Total Environ. 2017 Feb 15;580:258-265. doi: 10.1016/j.scitotenv.2016.12.081. Epub 2016 Dec 20.
Bacteria are abundant in atmospheric water phase with the potential to influence atmospheric processes and human health, yet relatively little information is known about the bacterial characteristics at high altitudes. Here we investigated the bacterial community by high throughput sequencing in 24 cloud water samples collected from September 26 to October 31, at the summit of Mt. Tai (36°15' N, 117°06' E, 1534m a.s.l) in China. Diverse bacterial population were identified and the gram-negative bacteria contributed the majority of total bacteria including Proteobacteria (81.6%) and Bacteroidetes (3.9%), followed by gram-positive bacteria Firmicutes (7.1%) and Actinobacteria (2.3%). These gram-negative taxa mainly inhabited in leaf-surface and cold environments. Meanwhile bacteria involved in the cloud condensation nuclei and ice nuclei formation were observed such as Sphingomonas (6.7%), Pseudomonas (4.1%), and Bacillus (1.1%). In addition, Sphingmonas was more active than that in daytime and participated in the cloud chemistry process. Meanwhile O and SO critically contributed to the variation of bacterial community. It is the first report on the bacterial community structure of cloud water over Asian area. Our results can serve as an important reference for environmental scientists, and biologists.
大气水相中存在丰富的细菌，它们具有影响大气过程和人类健康的潜力，但目前对于高海拔地区细菌特征的了解相对较少。本研究通过高通量测序，对 2018 年 9 月 26 日至 10 月 31 日在中国泰山山顶（36°15' N，117°06' E，1534m a.s.l.）采集的 24 个云水样中的细菌群落进行了调查。鉴定出了多样的细菌种群，其中革兰氏阴性菌在总细菌中占多数，包括变形菌门（81.6%）和拟杆菌门（3.9%），其次是革兰氏阳性菌厚壁菌门（Firmicutes，7.1%）和放线菌门（Actinobacteria，2.3%）。这些革兰氏阴性菌主要栖息在叶表面和寒冷环境中。同时还观察到参与云凝结核和冰核形成的细菌，如鞘氨醇单胞菌（Sphingomonas，6.7%）、假单胞菌（Pseudomonas，4.1%）和芽孢杆菌（Bacillus，1.1%）。此外，Sphingmonas 在夜间比白天更为活跃，并参与了云化学过程。同时 O 和 SO 对细菌群落的变化有重要贡献。这是亚洲地区云水中细菌群落结构的首次报道。我们的研究结果可为环境科学家和生物学家提供重要参考。
