Ma Yanhe, Zhang Weizhou, Xue Yanfen, Zhou Peijin, Ventosa Antonio, Grant William D
Department of Microbial Physiology and Ecology, Institute of Microbiology, Chinese Academy of Sciences, 100080, Beijing, China.
Extremophiles. 2004 Feb;8(1):45-51. doi: 10.1007/s00792-003-0358-z. Epub 2003 Oct 1.
Bacterial diversity associated with Baer Soda Lake in Inner Mongolia of China was investigated using a culture-independent method. Bacterial 16S rRNA gene libraries were generated using bacterial oligonucleotide primers, and 16S rRNA gene sequences of 58 clones were analyzed phylogenetically. The library was dominated by 16S rDNAs of Gram-negative bacteria (24% alpha-Proteobacteria, 31% beta-Proteobacteria, 33% gamma-Proteobacteria, and 2% delta-Proteobacteria), with a lower percentage of clones corresponding to Gram-positive bacteria. Forty cloned sequences were similar to that of known bacterial isolates (> 97% sequence similarity), represented by the species of the genera Brevundimonas, Comamonas, Alcaligenes, Stenotrophomonas, and Klebsiella. Eighteen cloned sequences showed less affiliation with known taxa (< 97% sequence similarity) and may represent novel taxa.
采用非培养方法对中国内蒙古巴尔苏打湖的细菌多样性进行了研究。使用细菌寡核苷酸引物构建细菌16S rRNA基因文库，并对58个克隆的16S rRNA基因序列进行了系统发育分析。该文库以革兰氏阴性菌的16S rDNA为主（α-变形菌纲占24%，β-变形菌纲占31%，γ-变形菌纲占33%，δ-变形菌纲占2%），对应革兰氏阳性菌的克隆比例较低。40个克隆序列与已知细菌分离株的序列相似（序列相似性>97%），以短波单胞菌属、丛毛单胞菌属、产碱杆菌属、嗜麦芽窄食单胞菌属和克雷伯菌属的物种为代表。18个克隆序列与已知分类群的亲缘关系较弱（序列相似性<97%），可能代表新的分类群。
