Lin S L, Zhao L H, Wang Y M, Dong S S, An D K
Yao Xue Xue Bao. 1989 Jan;24(1):48-52.
A normal phase liquid chromatographic method was developed for the determination of berberine-type alkaloids in rhizoma Coptidis and traditional Chinese medicine--Angong Niuhuang Wan. Experimental evidences indicate that a normal phase system of a silica column (3.9 x 250 mm) as stationary phase with an eluent of ethyl acetate-formic acid-ethanol (15:3:2) as mobile phase can separate excellently (R5 greater than 1.5) four berberine-type alkaloids in the samples tested. According to the results obtained with this method the percent content of berberine hydrochloride in some Angong Niuhuang Wan from different factories and different batches was 0.331-0.456% and the average recovery was 97.23%; coefficient of variation was 1.2%.
建立了一种正相液相色谱法，用于测定黄连及中成药——安宫牛黄丸中黄连素类生物碱的含量。实验证据表明，以硅胶柱（3.9×250mm）为固定相的正相系统，以乙酸乙酯-甲酸-乙醇（15:3:2）为流动相的洗脱液，能很好地分离（R5大于1.5）所测样品中的四种黄连素类生物碱。根据该方法得到的结果，不同厂家、不同批次的部分安宫牛黄丸中盐酸黄连素的含量为0.331-0.456%，平均回收率为97.23%；变异系数为1.2%。
