CD163+巨噬细胞在肿瘤基质中的预后影响以及CD8+ T细胞在浸润性肝外胆管癌癌细胞巢中的预后影响

Prognostic Impact of CD163+ Macrophages in Tumor Stroma and CD8+ T-Cells in Cancer Cell Nests in Invasive Extrahepatic Bile Duct Cancer.

作者信息

Miura Takuya, Yoshizawa Tadashi, Hirai Hideaki, Seino Hiroko, Morohashi Satoko, Wu Yunyan, Wakiya Taiichi, Kimura Norihisa, Kudo Daisuke, Ishido Keinosuke, Toyoki Yoshikazu, Kijima Hiroshi, Hakamada Kenichi

机构信息

Department of Gastroenterological Surgery, Hirosaki University Graduate School of Medicine, Hirosaki, Japan

Department of Pathology and Bioscience, Hirosaki University Graduate School of Medicine, Hirosaki, Japan.

出版信息

Anticancer Res. 2017 Jan;37(1):183-190. doi: 10.21873/anticanres.11304.

DOI:10.21873/anticanres.11304

PMID:28011489

Abstract

AIM

The aim of this study was to examine the clinicopathological influence of tumor-infiltrating cluster of differentiation (CD) 163 macrophages and CD8 T-cells, and to clarify the prognostic effects of these cells in patients with invasive extrahepatic bile duct cancer (EHBC).

MATERIALS AND METHODS

The numbers of CD8 T-cells in cancer cell nests and CD163 macrophages in tumor stroma were evaluated using immunohistochemistry in 101 resected EHBC specimens. Correlations with clinicopathological variables and overall survival were analyzed.

RESULTS

Perihilar EHBC and perineural invasion were significantly associated with a low number of tumor-infiltrating CD8 T-cells. Poorly- differentiated histology and nodal metastasis were significantly associated with a high number of tumor-infiltrating CD163 macrophages. A combination of high number of CD8 T-cells and low number of CD163 macrophages was independently related to better overall survival in the whole patient cohort (hazard ratio=0.127, p<0.001) and in patients treated with adjuvant chemotherapy (hazard ratio=0.139, p=0.021).

CONCLUSION

Infiltrating CD163 macrophages in tumor stroma and CD8 T-cells in cancer cell nests have a prognostic impact in patients with EHBC following resection and also after adjuvant chemotherapy.

摘要

目的

本研究旨在探讨肿瘤浸润性分化簇（CD）163巨噬细胞和CD8 T细胞对临床病理的影响，并阐明这些细胞对肝外胆管浸润癌（EHBC）患者的预后作用。

材料与方法

采用免疫组织化学方法对101例手术切除的EHBC标本中癌细胞巢内的CD8 T细胞数量和肿瘤基质中的CD163巨噬细胞数量进行评估。分析其与临床病理变量及总生存期的相关性。

结果

肝门部EHBC和神经周围浸润与肿瘤浸润性CD8 T细胞数量少显著相关。低分化组织学和淋巴结转移与肿瘤浸润性CD163巨噬细胞数量多显著相关。在整个患者队列中（风险比=0.127，p<0.001）以及接受辅助化疗的患者中（风险比=0.139，p=0.021），CD8 T细胞数量多和CD163巨噬细胞数量少的组合与更好的总生存期独立相关。