Suematsu M, Houzawa S, Miura S, Nagata H, Kitahora T, Morishita T, Oshio C, Tsuchiya M
Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan.
J Biolumin Chemilumin. 1989 Jul;4(1):531-4. doi: 10.1002/bio.1170040169.
Reaction difference of oxyradical generation and luminol-dependent photoemission of zymosan- and phorbol ester-treated neutrophils were investigated using a conventional photomultiplier and ultrasensitive photonic imaging technique. Zymosan-treated cells released a concentrated photonic burst corresponding to the cellular distribution. In contrast, phorbol ester-treated cells produced a negligible level of photoemission, and the additional application of Ca2+ ionophore enhanced the photonic burst, which was gradually spread out into extracellular space. Serine protease inhibitors did not attenuate PMA-induced chemiluminescence but did attenuate zymosan-induced chemiluminescence. This suggests the involvement of serine protease in the respiratory burst of phagocytizing neutrophils.
使用传统光电倍增管和超灵敏光子成像技术，研究了酵母聚糖和佛波酯处理的中性粒细胞中氧自由基生成和鲁米诺依赖性光发射的反应差异。酵母聚糖处理的细胞释放出与细胞分布相对应的集中光子爆发。相比之下，佛波酯处理的细胞产生的光发射水平可忽略不计，额外添加钙离子载体可增强光子爆发，并逐渐扩散到细胞外空间。丝氨酸蛋白酶抑制剂不会减弱佛波酯诱导的化学发光，但会减弱酵母聚糖诱导的化学发光。这表明丝氨酸蛋白酶参与了吞噬性中性粒细胞的呼吸爆发。
