Department of Diagnostic Immunology Research and Biochemistry, Roswell Park Memorial Institute, Buffalo, New York.
J Urol. 2017 Feb;197(2S):S148-S152. doi: 10.1016/j.juro.2016.10.100. Epub 2016 Dec 22.
Rabbit antiserum raised against the crude extract of normal human prostatic tissue contained antibodies to a prostatic tissue-specific antigen as shown by immunoprecipitation techniques. Using this antiserum a prostate antigen was detected in normal, benign hypertrophic, and malignant prostatic tissues, but not in other human tissues. The prostate antigen was purified to homogeneity from prostatic tissues and showed a single protein band on analytical polyacrylamide gel electrophoresis and isoelectric focusing. This report thus presents the first demonstration of the purification of a prostate-specific antigen that does not represent prostatic acid phosphatase.
兔抗正常人体前列腺组织粗提物的抗血清中含有抗前列腺组织特异性抗原的抗体，这一点通过免疫沉淀技术得到了证实。利用该抗血清，在正常、良性增生和恶性前列腺组织中检测到了一种前列腺抗原，但在其他人体组织中未检测到。该前列腺抗原从前列腺组织中被纯化至均一性，并在分析聚丙烯酰胺凝胶电泳和等电聚焦中显示出单一的蛋白质条带。因此，本报告首次证明了一种前列腺特异性抗原的纯化，该抗原不代表前列腺酸性磷酸酶。
