Richard K A, Speziale S C, Staite N D, Berger A E, Deibel M R, Finzel B C, Einspahr H M
Department of Hypersensitivity Diseases, Upjohn Company, Kalamazoo, MI 49001.
Agents Actions. 1989 Jun;27(3-4):265-7. doi: 10.1007/BF01972792.
Soybean trypsin inhibitor (SBTI) shares some structural homology with interleukin-1 (IL-1) and was tested for IL-1 bioactivity. Human T-cells proliferated maximally when stimulated with PMA and SBTI but failed to respond to either stimulus alone. This response was abrogated by neutralizing antibodies to IL-1 beta but not to IL-1 alpha. However, immunoblots showed no cross-reactivity between SBTI and anti-IL-1 antibodies. Furthermore, SBTI did not bind to IL-1 receptors on YT cells and did not activate a murine T-lymphoma or human T-hybridoma. Supernantants from monocytes stimulated with SBTI contained significant levels of IL-1 activity. The data show that SBTI has no direct IL-1 activity but can stimulate T-cells indirectly through an IL-1 dependent mechanism.
大豆胰蛋白酶抑制剂（SBTI）与白细胞介素-1（IL-1）具有一些结构同源性，并对其IL-1生物活性进行了测试。当用人佛波酯（PMA）和SBTI刺激时，人T细胞增殖达到最大值，但单独对任何一种刺激均无反应。这种反应被抗IL-1β的中和抗体消除，但抗IL-1α的中和抗体则不能。然而，免疫印迹显示SBTI与抗IL-1抗体之间没有交叉反应。此外，SBTI不与YT细胞上的IL-1受体结合，也不激活鼠T淋巴瘤或人T杂交瘤。用SBTI刺激的单核细胞培养上清液含有显著水平的IL-1活性。数据表明，SBTI没有直接的IL-1活性，但可以通过IL-1依赖性机制间接刺激T细胞。
