Global Stem cell Technology NV, Noorwegenstraat 4, 9940, Evergem, Belgium.
Department of Medical Imaging and Orthopedics of Domestic Animals, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820, Merelbeke, Belgium.
Stem Cell Res Ther. 2021 Jul 13;12(1):393. doi: 10.1186/s13287-021-02457-9.
Mesenchymal stem cell treatments in dogs have been investigated as a potential innovative alternative to current conventional therapies for a variety of conditions. So far, the precise mode of action of the MSCs has yet to be determined. The aim of this study was to gain more insights into the pharmacokinetics of MSCs by evaluating their biodistribution in healthy dogs after different injection routes.
Three different studies were performed in healthy dogs to evaluate the biodistribution pattern of radiolabelled equine peripheral blood-derived mesenchymal stem cells following intravenous, intramuscular and subcutaneous administration in comparison with free Technetium. The labelling of the equine peripheral blood-derived mesenchymal stem cells was performed using stannous chloride as a reducing agent. Whole-body scans were obtained using a gamma camera during a 24-h follow-up.
The labelling efficiency ranged between 59.58 and 83.82%. Free Technetium accumulation was predominantly observed in the stomach, thyroid, bladder and salivary glands, while following intravenous injection, the Technetium-labelled equine peripheral blood-derived mesenchymal stem cells majorly accumulated in the liver throughout the follow-up period. After intramuscular and subcutaneous injection, the injected dose percentage remained very high at the injection site.
A distinct difference was noted in the biodistribution pattern of the radiolabelled equine peripheral blood-derived mesenchymal stem cells compared to free Technetium indicating equine peripheral blood-derived mesenchymal stem cells have a specific pharmacokinetic pattern after systemic administration in healthy dogs. Furthermore, the biodistribution pattern of the used xenogeneic equine peripheral blood-derived mesenchymal stem cells appeared to be different from previously reported experiments using different sources of mesenchymal stem cells.
间充质干细胞治疗已被研究为一种有潜力的创新替代方法，可用于治疗多种疾病，替代目前的常规疗法。到目前为止，间充质干细胞的确切作用机制尚未确定。本研究的目的是通过评估不同注射途径后健康犬中的生物分布，进一步了解间充质干细胞的药代动力学。
在健康犬中进行了三项不同的研究，以评估放射性标记的马外周血源性间充质干细胞在静脉、肌肉内和皮下注射后的生物分布模式，并与游离锝进行比较。使用氯化亚锡作为还原剂对马外周血源性间充质干细胞进行标记。在 24 小时的随访过程中，使用伽马相机进行全身扫描。
标记效率在 59.58%至 83.82%之间。游离锝主要在胃、甲状腺、膀胱和唾液腺中积聚，而静脉注射后，整个随访过程中，锝标记的马外周血源性间充质干细胞主要在肝脏中积聚。肌肉内和皮下注射后，注射部位的注射剂量百分比仍非常高。
与游离锝相比，放射性标记的马外周血源性间充质干细胞的生物分布模式有明显差异，表明健康犬全身给药后，马外周血源性间充质干细胞具有特定的药代动力学模式。此外，所使用的异种马外周血源性间充质干细胞的生物分布模式似乎与之前使用不同来源的间充质干细胞进行的实验不同。
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