兔软骨细胞中的磷脂酶A2（PLA2）活性
Phospholipase A2 (PLA2) activity in rabbit chondrocytes.
作者信息
Stevens T M, Chin J E, McGowan M, Giannaras J, Kerr J S
机构信息
medical Products Department, E. I. Du Pont de Nemours & Co., Inc., Wilmington, DE 19880-0400.
出版信息
Agents Actions. 1989 Jun;27(3-4):385-7. doi: 10.1007/BF01972829.
The effects of recombinant interleukin-1 beta (rIL-1 beta), recombinant tumor necrosis factor (rTNF alpha) and two growth factors, basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) on PLA2 activity and prostaglandin E2 (PGE2) release were investigated using rabbit chondrocytes. Cellular PLA2 activity increased 2-10 x above controls in the presence of 8 x 10(-12) M (5 U) rIL-1 beta or 5 x 10(-9) M rTNF alpha after 20 hr incubation. PLA2 activity remained constant with 1-50 ng/ml of either growth factor. PGE2 release significantly increased (p less than 0.05) when the chondrocytes were incubated with rIL-1 beta, bFGF and EGF alone, but not with rTNF alpha above. These data suggest PLA2 activity and PGE2 release are not coordinately regulated in rabbit chondrocytes.
使用兔软骨细胞研究了重组白细胞介素-1β（rIL-1β）、重组肿瘤坏死因子（rTNFα）以及两种生长因子，即碱性成纤维细胞生长因子（bFGF）和表皮生长因子（EGF）对磷脂酶A2（PLA2）活性和前列腺素E2（PGE2）释放的影响。在8×10⁻¹² M（5 U）rIL-1β或5×10⁻⁹ M rTNFα存在的情况下，孵育20小时后，细胞PLA2活性比对照增加2至10倍。两种生长因子浓度在1至50 ng/ml时，PLA2活性保持恒定。当软骨细胞单独与rIL-1β、bFGF和EGF孵育时，PGE2释放显著增加（p < 0.05），但与上述rTNFα孵育时则不然。这些数据表明，兔软骨细胞中PLA2活性和PGE2释放并非协同调节。
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