Kerr J S, Stevens T M, Davis G L, McLaughlin J A, Harris R R
Medical Products Department, E. I. Du Pont de Nemours & Co. (Inc.), Wilmington, DE 19880-0400.
Biochem Biophys Res Commun. 1989 Dec 29;165(3):1079-84. doi: 10.1016/0006-291x(89)92712-5.
We have investigated the effects of recombinant interleukin-1 beta (rIL-1 beta) on phospholipase A2 activity (PLA2), PLA2 messenger RNA levels, and eicosanoid production in rabbit chondrocytes. Phospholipase A2 activity increased 5 fold with exposure to 1.6 x 10(-11) M rIL-1 beta for 20 hours. An mRNA specific for an extracellular PLA2 was detected by RNA blot hybridization after treatment of the cells with rIL-1 beta. Hydroxylated derivatives of arachidonic acid, including prostaglandins, leukotriene B4, 5-hydroxyeicosa 6E, 8Z, 11Z, 14Z-tetraenoic acid, 12-hydroxyeicosa 5Z, 8Z, 10E, 14Z-tetraenoic acid and 15-hydroxyeicosa 5Z, 8Z, 11Z, 13E-tetraenoic acid increased in cells after rIL-1 beta treatment.
我们研究了重组白细胞介素-1β（rIL-1β）对兔软骨细胞中磷脂酶A2活性（PLA2）、PLA2信使核糖核酸水平以及类花生酸生成的影响。暴露于1.6×10⁻¹¹ M rIL-1β 20小时后，磷脂酶A2活性增加了5倍。在用rIL-1β处理细胞后，通过RNA印迹杂交检测到一种细胞外PLA2的特异性信使核糖核酸。rIL-1β处理后，细胞中花生四烯酸的羟基化衍生物增加，包括前列腺素、白三烯B4、5-羟基二十碳6E,8Z,11Z,14Z-四烯酸、12-羟基二十碳5Z,8Z,10E,14Z-四烯酸和15-羟基二十碳5Z,8Z,11Z,13E-四烯酸。
