Lyons-Giordano B, Davis G L, Galbraith W, Pratta M A, Arner E C
Medical Products Department E. I. Du Pont de Nemours and Company, Inc., Wilmington, DE 19880-0400.
Biochem Biophys Res Commun. 1989 Oct 16;164(1):488-95. doi: 10.1016/0006-291x(89)91746-4.
A cDNA that codes for human rheumatoid synovial fluid phospholipase A2 (PLA2) hybridized with a RNA of the same size (900 base pairs) isolated from rabbit articular chondrocytes (RAC). Treatment of RAC with 100 ng/ml recombinant human interleukin-1 beta (IL-1) for 24 hours caused a 13-fold increase in mRNA for this PLA2. Timecourse studies demonstrated that maximal induction of PLA2 mRNA occurred by 16 hours post addition of IL-1 (100 ng/ml). Augmentation of RAC PLA2 mRNA levels was dose-dependent; half-maximal induction was estimated to require 0.15 ng/ml IL-1. Actinomycin D inhibited IL-1 effects on PLA2 mRNA levels. Coordinate effects of IL-1 on RAC PLA2 activity were observed with respect to time and dose dependence as well as actinomycin D sensitivity.
一种编码人类风湿性滑液磷脂酶A2（PLA2）的cDNA与从兔关节软骨细胞（RAC）分离出的相同大小（900个碱基对）的RNA杂交。用100 ng/ml重组人白细胞介素-1β（IL-1）处理RAC 24小时，导致这种PLA2的mRNA增加了13倍。时间进程研究表明，在添加IL-1（100 ng/ml）后16小时，PLA2 mRNA出现最大诱导。RAC中PLA2 mRNA水平的增加呈剂量依赖性；估计半最大诱导需要0.15 ng/ml IL-1。放线菌素D抑制IL-1对PLA2 mRNA水平的影响。观察到IL-1对RAC中PLA2活性的时间和剂量依赖性以及放线菌素D敏感性的协同作用。
