Liu Li, Ning Bingbing, Cui Jingang, Zhang Teng, Chen Yu
Yueyang Hospital & Clinical Research Institute of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.
Yueyang Hospital & Clinical Research Institute of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.
J Ethnopharmacol. 2017 Feb 23;198:1-4. doi: 10.1016/j.jep.2016.12.036. Epub 2016 Dec 22.
Panax notoginseng (Burkill) F.H. Chen (Araliaceae) has a long history of clinical application in China for the treatment of cardiovascular diseases. Panax notoginseng saponins (PNS) have been proven to be the major cardioprotective substances of Panax notoginseng (Burkill) F.H. Chen (Araliaceae).
The current study further investigated the molecular mechanisms associated with the cardioprotective effect of PNS.
C57BL/6J mice were subject to isoproterenol (ISO)-induced myocardial injury in the absence or presence of PNS treatment. Histological, immunohistochemical and molecular biological approaches were taken to assess the effects of PNS treatment on ISO-induced myocardial injury and ensuing fibrogenesis.
PNS treatment significantly attenuated ISO-induced myocardial injury and fibrosis. The expression of an anti-fibrotic microRNA, miR-29c, was significantly decreased in ISO-challenged mouse hearts. In contrast, PNS treatment resulted in increased cardiac expression of miR-29c. The expression of miR-29c target genes including Collagen (Col) 1a1, Col1a2, Col3a1 and Col5a1, fibrillin 1 (Fbn1) as well as TGFβ1 was significantly increased by ISO, which exhibited decreased expression by PNS intervention.
Our results demonstrate for the first time that the cardioprotective effects of PNS could in part implicate increased expression of miR-29c in the heart, which may help increase the understanding of the pharmacological activities of PNS in treating cardiovascular disorders.
三七（五加科）在中国治疗心血管疾病有着悠久的临床应用历史。三七皂苷（PNS）已被证明是三七的主要心脏保护物质。
本研究进一步探讨了PNS心脏保护作用的分子机制。
C57BL/6J小鼠在接受或不接受PNS治疗的情况下，用异丙肾上腺素（ISO）诱导心肌损伤。采用组织学、免疫组织化学和分子生物学方法评估PNS治疗对ISO诱导的心肌损伤及随后纤维化的影响。
PNS治疗显著减轻了ISO诱导的心肌损伤和纤维化。在受ISO攻击的小鼠心脏中，抗纤维化微小RNA miR-29c的表达显著降低。相反，PNS治疗导致心脏中miR-29c的表达增加。ISO显著增加了miR-29c靶基因的表达，包括胶原蛋白（Col）1a1、Col1a2、Col3a1和Col5a1、原纤蛋白1（Fbn1）以及TGFβ1，而PNS干预使其表达降低。
我们的结果首次证明，PNS的心脏保护作用可能部分与心脏中miR-29c表达增加有关，这可能有助于加深对PNS治疗心血管疾病药理活性的理解。
