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使用WST-1在平板试验中测定超氧化物歧化酶活性。

Assay of superoxide dismutase activity in a plate assay using WST-1.

作者信息

Peskin Alexander V, Winterbourn Christine C

机构信息

Centre for Free Radical Research, Department of Pathology, University of Otago Christchurch, Christchurch, New Zealand.

Centre for Free Radical Research, Department of Pathology, University of Otago Christchurch, Christchurch, New Zealand.

出版信息

Free Radic Biol Med. 2017 Feb;103:188-191. doi: 10.1016/j.freeradbiomed.2016.12.033. Epub 2016 Dec 23.

DOI:10.1016/j.freeradbiomed.2016.12.033
PMID:28017897
Abstract

One of the most convenient methods for measuring superoxide dismutase activity is a plate assay using xanthine oxidase and the water soluble tetrazolium WST-1. For reliable results with WST-1, certain aspects of the procedure need to be adhered to. This article describes an appropriate protocol that minimizes sources of variability.

摘要

测量超氧化物歧化酶活性最便捷的方法之一是使用黄嘌呤氧化酶和水溶性四氮唑WST-1进行平板测定。为了获得使用WST-1的可靠结果，需要遵循该程序的某些方面。本文介绍了一种适当的方案，可将变异性来源降至最低。

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