Loss Raquel A, Pereira Gabriela N, Boschetto Daiane L, Aguiar Gean S P, Machado Juliana R, Chaves Lorenzo M P C, Silva Maria J A, Oliveira Débora, Oliveira J Vladimir
Department of Chemical and Food Engineering, UFSC, Florianópolis, SC Brazil ; Universidade Estadual do Estado de Mato Grosso, Barra do Bugres, MT 78390-000 Brazil.
Department of Chemical and Food Engineering, UFSC, Florianópolis, SC Brazil.
J Food Sci Technol. 2016 Oct;53(10):3859-3864. doi: 10.1007/s13197-016-2362-1. Epub 2016 Oct 17.
Eugenyl acetate obtained via enzymatic esterification using Lipozyme TL IM enzyme was encapsulated in biopolymer poly(3-hydroxybutyrate-co-hydroxyvalerate) (PHBV) through solution-enhanced dispersion by supercritical fluids (SEDS). Produced particles were characterized by SEM and confocal microscopy techniques and in addition in vitro release assays were performed in isopropanol and ethyl acetate. Experimental micronization conditions comprised 8 and 10 MPa, 308 and 313 K and eugenyl acetate concentration ranging from 5 to 20 mg mL, keeping PHBV concentration constant (20 mg mL in dichloromethane). The maximum encapsulation efficiency was 58.0 % for 5 mg mLof eugenyl acetate at 8 MPa and 308 K. The morphology of the encapsulated particles for most of the trials was spherical, with particle size ranging from 0.061 to 0.276 μm. Regarding the release in ethyl acetate and isopropanol solvents the higher the affinity of the encapsulated ester of these solvents, the faster the release was observed. These results demonstrate the importance of essential clove oil esterification reaction and encapsulation of the ester by SEDS method so that this encapsulated ester can be used in different industrial applications.
通过使用Lipozyme TL IM酶进行酶促酯化反应得到的乙酸丁香酯，采用超临界流体增强溶液分散法（SEDS）被包封在生物聚合物聚（3-羟基丁酸酯-co-羟基戊酸酯）（PHBV）中。通过扫描电子显微镜（SEM）和共聚焦显微镜技术对制备的颗粒进行表征，此外，还在异丙醇和乙酸乙酯中进行了体外释放试验。实验微粉化条件包括8和10兆帕、308和313开尔文，乙酸丁香酯浓度范围为5至20毫克/毫升，同时保持PHBV浓度恒定（在二氯甲烷中为20毫克/毫升）。在8兆帕和308开尔文条件下，对于5毫克/毫升的乙酸丁香酯，最大包封效率为58.0%。在大多数试验中，包封颗粒的形态为球形，粒径范围为0.061至0.276微米。关于在乙酸乙酯和异丙醇溶剂中的释放情况，这些溶剂与包封酯的亲和力越高，释放速度越快。这些结果证明了丁香精油酯化反应以及通过SEDS方法对酯进行包封的重要性，以便这种包封酯能够用于不同的工业应用。
