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用于骨科修复应用的脂肪来源间充质干细胞活力及分化特性：脂肪组织库

Adipose Derived-Mesenchymal Stem Cells Viability and Differentiating Features for Orthopaedic Reparative Applications: Banking of Adipose Tissue.

作者信息

Roato Ilaria, Alotto Daniela, Belisario Dimas Carolina, Casarin Stefania, Fumagalli Mara, Cambieri Irene, Piana Raimondo, Stella Maurizio, Ferracini Riccardo, Castagnoli Carlotta

机构信息

CeRMS, A.O.U. Città della Salute e della Scienza, Torino, Italy.

Skin Bank, Department of General and Specialized Surgery, A.O.U. Città della Salute e della Scienza, Torino, Italy.

出版信息

Stem Cells Int. 2016;2016:4968724. doi: 10.1155/2016/4968724. Epub 2016 Nov 29.

DOI:10.1155/2016/4968724
PMID:28018432
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5153503/
Abstract

Osteoarthritis is characterized by loss of articular cartilage also due to reduced chondrogenic activity of mesenchymal stem cells (MSCs) from patients. Adipose tissue is an attractive source of MSCs (ATD-MSCs), representing an effective tool for reparative medicine, particularly for treatment of osteoarthritis, due to their chondrogenic and osteogenic differentiation capability. The treatment of symptomatic knee arthritis with ATD-MSCs proved effective with a single infusion, but multiple infusions could be also more efficacious. Here we studied some crucial aspects of adipose tissue banking procedures, evaluating ATD-MSCs viability, and differentiation capability after cryopreservation, to guarantee the quality of the tissue for multiple infusions. We reported that the presence of local anesthetic during lipoaspiration negatively affects cell viability of cryopreserved adipose tissue and cell growth of ATD-MSCs in culture. We observed that DMSO guarantees a faster growth of ATD-MSCs in culture than trehalose. At last, ATD-MSCs derived from fresh and cryopreserved samples at -80°C and -196°C showed viability and differentiation ability comparable to fresh samples. These data indicate that cryopreservation of adipose tissue at -80°C and -196°C is equivalent and preserves the content of ATD-MSCs in Stromal Vascular Fraction (SVF), guaranteeing the differentiation ability of ATD-MSCs.

摘要

骨关节炎的特征还在于关节软骨的丧失，这也是由于患者间充质干细胞（MSC）的软骨生成活性降低所致。脂肪组织是一种有吸引力的间充质干细胞来源（脂肪组织来源的间充质干细胞，ATD-MSCs），由于其软骨生成和成骨分化能力，它是再生医学的一种有效工具，尤其适用于骨关节炎的治疗。用ATD-MSCs治疗有症状的膝关节炎，单次输注已证明有效，但多次输注可能更有效。在此，我们研究了脂肪组织储存程序的一些关键方面，评估了冷冻保存后ATD-MSCs的活力和分化能力，以确保多次输注时组织的质量。我们报告称，抽脂过程中局部麻醉剂的存在会对冷冻保存的脂肪组织的细胞活力以及培养中ATD-MSCs的细胞生长产生负面影响。我们观察到，在培养中，二甲基亚砜（DMSO）能保证ATD-MSCs比海藻糖生长得更快。最后，来自新鲜样本以及在-80°C和-196°C下冷冻保存样本的ATD-MSCs显示出与新鲜样本相当的活力和分化能力。这些数据表明，在-80°C和-196°C下对脂肪组织进行冷冻保存是等效的，并且能保留基质血管成分（SVF）中ATD-MSCs的含量，确保ATD-MSCs的分化能力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52f5/5153503/7d23f4df7398/SCI2016-4968724.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52f5/5153503/02812f0fe39a/SCI2016-4968724.001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52f5/5153503/35d6480ae0cf/SCI2016-4968724.003.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52f5/5153503/57803b69123b/SCI2016-4968724.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52f5/5153503/a7a3b743c339/SCI2016-4968724.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52f5/5153503/7d23f4df7398/SCI2016-4968724.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52f5/5153503/02812f0fe39a/SCI2016-4968724.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52f5/5153503/9dbc51be014d/SCI2016-4968724.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52f5/5153503/35d6480ae0cf/SCI2016-4968724.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52f5/5153503/58830c2b2c8a/SCI2016-4968724.004.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52f5/5153503/7d23f4df7398/SCI2016-4968724.007.jpg

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