使用光片显微镜对早期胚胎心脏进行体积光学映射。
Volumetric optical mapping in early embryonic hearts using light-sheet microscopy.
作者信息
Ma Pei, Chan Dennis C, Gu Shi, Watanabe Michiko, Jenkins Michael W, Rollins Andrew M
机构信息
Department of Biomedical Engineering, Case Western Reserve University, Cleveland, Ohio 44106, USA; Shanghai Key Laboratory of Modern Optical Systems, College of Optical-Electrical and Computer Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China.
Department of Biomedical Engineering, Case Western Reserve University, Cleveland, Ohio 44106, USA.
出版信息
Biomed Opt Express. 2016 Nov 15;7(12):5120-5128. doi: 10.1364/BOE.7.005120. eCollection 2016 Dec 1.
Optical mapping (OM) of electrical activity using voltage-sensitive fluorescent dyes is a powerful tool for the investigation of embryonic cardiac electrophysiology. However, because conventional OM integrates the signal in depth and projects it to a two-dimensional plane, information acquired is incomplete and dependent upon the orientation of the sample. This complicates interpretation of data, especially when comparing one heart to another. To overcome this limitation, we present volumetric OM using light-sheet microscopy, which enables high-speed capture of optically sectioned slices. Voltage-sensitive fluorescence images from multiple planes across entire early embryonic quail hearts were acquired, and complete, orientation-independent, four-dimensional maps of transmembrane potential are demonstrated. Volumetric OM data were collected while using optical pacing to control the heart rate, paving the way for physiological measurements and precise manipulation of the heartbeat in the future.
使用电压敏感染料对电活动进行光学映射（OM）是研究胚胎心脏电生理学的有力工具。然而，由于传统的OM对信号进行深度积分并将其投影到二维平面上，所获取的信息不完整且依赖于样本的方向。这使得数据的解释变得复杂，尤其是在比较不同心脏时。为了克服这一限制，我们展示了使用光片显微镜的体积OM，它能够高速捕获光学切片。获取了整个早期胚胎鹌鹑心脏多个平面的电压敏感染荧光图像，并展示了完整的、与方向无关的跨膜电位四维图谱。在使用光学起搏控制心率的同时收集了体积OM数据，为未来的生理测量和心跳的精确操纵铺平了道路。
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