Ladejobi Olufunmilayo, Elderfield James, Gardner Keith A, Gaynor R Chris, Hickey John, Hibberd Julian M, Mackay Ian J, Bentley Alison R
The John Bingham Laboratory, NIAB, Huntingdon Road, Cambridge CB3 0LE, United Kingdom; Department of Plant Sciences, The University of Cambridge, Downing Street, Cambridge CB2 3EA, United Kingdom.
Department of Plant Sciences, The University of Cambridge, Downing Street, Cambridge CB2 3EA, United Kingdom.
Appl Transl Genom. 2016 Oct 26;11:9-17. doi: 10.1016/j.atg.2016.10.002. eCollection 2016 Dec.
Most agriculturally significant crop traits are quantitatively inherited which limits the ease and efficiency of trait dissection. Multi-parent populations overcome the limitations of traditional trait mapping and offer new potential to accurately define the genetic basis of complex crop traits. The increasing popularity and use of nested association mapping (NAM) and multi-parent advanced generation intercross (MAGIC) populations raises questions about the optimal design and allocation of resources in their creation. In this paper we review strategies for the creation of multi-parent populations and describe two complementary studies addressing the design and construction of NAM and MAGIC populations. The first simulates the selection of diverse founder parents and the second the influence of multi-parent crossing schemes (and number of founders) on haplotype creation and diversity. We present and apply two open software resources to simulate alternate strategies for the development of multi-parent populations.
大多数具有农业重要性的作物性状是数量遗传的，这限制了性状剖析的简便性和效率。多亲本群体克服了传统性状定位的局限性，并为准确界定复杂作物性状的遗传基础提供了新的潜力。巢式关联作图（NAM）群体和多亲本高代杂交（MAGIC）群体越来越受欢迎且应用广泛，这引发了关于其创建过程中资源的最优设计和分配的问题。在本文中，我们综述了创建多亲本群体的策略，并描述了两项补充研究，它们涉及NAM群体和MAGIC群体的设计与构建。第一项研究模拟了不同奠基亲本的选择，第二项研究模拟了多亲本杂交方案（以及奠基亲本的数量）对单倍型创建和多样性的影响。我们展示并应用了两个开放软件资源，以模拟多亲本群体开发的替代策略。
