Hernández-Caselles T, Vera A, Crespo F, Villalaín J, Gómez-Fernández J C
Departamento de Bioquímica, Facultad de Veterinaria, Universidad de Murcia, Spain.
Am J Vet Res. 1989 Sep;50(9):1486-8.
Liposomes with entrapped gentamicin were used to treat mice with infection attributable to Brucella melitensis. Liposomes bearing positive charge and formed by egg yolk lecithin, cholesterol, and stearylamine were effective in the elimination of B melitensis residing in liver and spleen. Negatively charged liposomes, formed by egg yolk lecithin, cholesterol, and dicetyl phosphate were also effective in suppression of the infection in liver, but were less so in suppression of the infection in the spleen. Free gentamicin was less effective than the encapsulated antibiotic. At 20 hours after administration of gentamicin encapsulated in liposomes, the gentamicin concentrations in liver and spleen were similar, regardless of the charge of the liposomes--neutral, positive, or negative. However, positively charged liposomes were more efficient than were other liposome types for the treatment of brucellosis caused by B melitensis.
包裹庆大霉素的脂质体被用于治疗由羊布鲁氏菌引起感染的小鼠。由蛋黄卵磷脂、胆固醇和硬脂胺形成的带正电荷的脂质体，在消除肝脏和脾脏中的羊布鲁氏菌方面是有效的。由蛋黄卵磷脂、胆固醇和磷酸二鲸蜡酯形成的带负电荷的脂质体，在抑制肝脏感染方面也有效，但在抑制脾脏感染方面效果较差。游离庆大霉素的效果不如包裹的抗生素。在给予脂质体包裹的庆大霉素20小时后，无论脂质体的电荷是中性、正性还是负性，肝脏和脾脏中的庆大霉素浓度相似。然而，带正电荷的脂质体在治疗由羊布鲁氏菌引起的布鲁氏菌病方面比其他类型的脂质体更有效。
