利用线粒体 DNA 细胞色素 b 区分析来自印度九条河流的野生鲤鱼群体的遗传分化。
Genetic divergence in wild population of Labeo rohita (Hamilton, 1822) from nine Indian rivers, analyzed through MtDNA cytochrome b region.
机构信息
National Bureau of Fish Genetic Resources (ICAR), Canal Ring Road, P.O. Dilkusha, Lucknow, 226 002, Uttar Pradesh, India.
出版信息
Mol Biol Rep. 2012 Apr;39(4):3659-65. doi: 10.1007/s11033-011-1140-4. Epub 2011 Jul 7.
The present study examined partial cytochrome b gene sequence of mitochondrial DNA for polymorphism and its suitability to determine the genetic differentiation in wild Labeo rohita. The 146 samples of L. rohita were collected from nine distant rivers; Satluj, Brahmaputra, Son, Chambal Mahanadi, Rapti, Chauka, Bhagirathi and Tons were analyzed. Sequencing of 307 bp of Cyto b gene revealed 35 haplotypes with haplotype diversity 0.751 and nucleotide diversity (π) 0.005. The within population variation accounted for 84.21% of total variation and 15.79% was found to among population. The total Fst value, 0.158 (P < 0.05) was found to be significant. The results concluded that the partial cyto b is polymorphic and can be a potential marker to determining genetic stock structure of L. rohita wild population.
本研究检测了线粒体 DNA 细胞色素 b 基因部分序列的多态性及其在确定野生罗非鱼遗传分化方面的适用性。从 Satluj、Brahmaputra、Son、Chambal Mahanadi、Rapti、Chauka、Bhagirathi 和 Tons 九条不同的河流中采集了 146 个罗非鱼样本进行分析。对 307 bp 的 Cyto b 基因进行测序，发现了 35 种单倍型，单倍型多样性为 0.751，核苷酸多样性（π）为 0.005。种群内变异占总变异的 84.21%，而种群间变异占 15.79%。总 Fst 值为 0.158（P<0.05），具有显著意义。研究结果表明，部分细胞色素 b 具有多态性，可作为确定野生罗非鱼种群遗传结构的潜在标记。
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