Department of Chemistry, Hindustan Institute of Technology and Science, Padur, Chennai 603 103, India.
Department of Chemistry, Hindustan Institute of Technology and Science, Padur, Chennai 603 103, India.
Carbohydr Polym. 2017 Feb 20;158:51-57. doi: 10.1016/j.carbpol.2016.11.092. Epub 2016 Dec 2.
A novel type of ethylene glycol dimethacrylate (EGDMA) cross-linked guar gum oleate-graft-poly(methacrylic acid) (GGO-g-PMAc) hydrogel was prepared as a pH-responsive controlled release carrier for colon-specific drug delivery. The structure of GGO-g-PMAc hydrogel was characterized by FT-IR, H NMR and X-ray diffraction (XRD) analysis. The swelling degree of the GGO-g-PMAc hydrogel at pH 7.4 was found to be higher than that at pH 1.2. The drug release studies performed in pH 7.4 and 1.2 buffer solutions at 37°C revealed that the rate and amount of drug released from the GGO-g-PMAc hydrogel at pH 7.4 were higher than that at pH 1.2. The MTT assay revealed that there is no noticeable cytotoxicity of GGO-g-PMAc hydrogel at the concentration range of 0-100μg/ml against the mouse mesenchymal stem cell line (C3H10T1/2). These results suggested that GGO-g-PMAc hydrogel can be a prospective pH-sensitive carrier for colon-targeted drug delivery.
一种新型的乙二醇二甲基丙烯酸酯（EGDMA）交联瓜尔胶油酸接枝-聚（甲基丙烯酸）（GGO-g-PMAc）水凝胶被制备为用于结肠特异性药物传递的 pH 响应性控制释放载体。通过傅里叶变换红外光谱（FT-IR）、核磁共振（H NMR）和 X 射线衍射（XRD）分析对 GGO-g-PMAc 水凝胶的结构进行了表征。在 pH 7.4 时，GGO-g-PMAc 水凝胶的溶胀度高于在 pH 1.2 时的溶胀度。在 37°C 下在 pH 7.4 和 1.2 缓冲溶液中进行的药物释放研究表明，在 pH 7.4 时从 GGO-g-PMAc 水凝胶中释放的药物的速率和数量高于在 pH 1.2 时的速率和数量。MTT 测定表明，在 0-100μg/ml 浓度范围内，GGO-g-PMAc 水凝胶对小鼠间充质干细胞系（C3H10T1/2）没有明显的细胞毒性。这些结果表明，GGO-g-PMAc 水凝胶可以作为一种有前途的 pH 敏感的结肠靶向药物传递载体。
