Li Yumei, Li Xiang, Zhao Rui, Wang Chuying, Qiu Fangping, Sun Bolun, Ji He, Qiu Ju, Wang Ce
Alan G. MacDiarmid Institute, Jilin University, Changchun 130012, People's Republic of China; Department of Clinical Pharmacy and Traditional Chinese Medicine Pharmacology, School of Pharmaceutical Sciences, Changchun University of Chinese Medicine, Changchun 130117, People's Republic of China.
Alan G. MacDiarmid Institute, Jilin University, Changchun 130012, People's Republic of China.
Mater Sci Eng C Mater Biol Appl. 2017 Mar 1;72:106-112. doi: 10.1016/j.msec.2016.11.052. Epub 2016 Nov 16.
Recently, electrically conductive biomaterial scaffolds have shown great potential in tissue regeneration. Herein, we reported an electrically conductive polyaniline (PANI) coated poly(ε-caprolactone) (PCL) electrospun micron-fiber scaffold for the enhanced attachment and proliferation of human umbilical vein endothelial cells (HUVECs) under electrical stimulation conditions. After the O plasma treatment toward PCL electrospun fiber, PANI could be polymerized onto their surfaces successfully. The obtained PANI-PCL fibers were characterized by SEM observations, FT-IR spectra, XPS analysis, and water contact angle measurement. The mechanical tests indicated that the fibers could satisfy the practical vascular scaffold requirements. The conductivity of the PANI-PCL fibers was 6.71×10S/cm which could provide a conductive in-vitro platform to study the effect of electrical stimulation on HUVECs proliferation. When PANI-coated PCL fibers were compared with PCL fibers, HUVECs exhibited highly enhanced adhesion and viability, especially under electrical stimulation (ES) of 200, 300, and 400mV/cm. Proliferation of HUVECs on PANI-PCL fibers was strongly dependent on electrical stimulation intensity. The results showed new insights into conductive scaffolds for vascular tissue engineering.
最近，导电生物材料支架在组织再生方面显示出巨大潜力。在此，我们报道了一种导电聚苯胺（PANI）涂层的聚（ε-己内酯）（PCL）电纺微米纤维支架，用于在电刺激条件下增强人脐静脉内皮细胞（HUVECs）的附着和增殖。对PCL电纺纤维进行O等离子体处理后，PANI可成功聚合到其表面。通过扫描电子显微镜（SEM）观察、傅里叶变换红外光谱（FT-IR）、X射线光电子能谱（XPS）分析和水接触角测量对所得的PANI-PCL纤维进行了表征。力学测试表明，这些纤维能够满足实际血管支架的要求。PANI-PCL纤维的电导率为6.71×10S/cm，可为研究电刺激对HUVECs增殖的影响提供一个导电体外平台。当将PANI涂层的PCL纤维与PCL纤维进行比较时，HUVECs表现出高度增强的附着力和活力，尤其是在200、300和400mV/cm的电刺激（ES）下。HUVECs在PANI-PCL纤维上的增殖强烈依赖于电刺激强度。这些结果为血管组织工程的导电支架提供了新的见解。
