Qiu Weiwei, Zhu Qionghua, Gao Fei, Gao Feng, Huang Jiafu, Pan Yutian, Wang Qingxiang
College of Chemistry and Environment, Fujian Province Key Laboratory of Morden Analytical Science and Separation Technology, Minnan Normal University, Zhangzhou 363000, PR China.
College of Biological Science and Technology, Minnan Normal University, Zhangzhou 363000, PR China.
Mater Sci Eng C Mater Biol Appl. 2017 Mar 1;72:692-700. doi: 10.1016/j.msec.2016.11.134. Epub 2016 Dec 5.
A novel electrochemical non-enzymatic hydrogen peroxide (HO) sensor has been developed based on Prussian blue (PB) and electrochemically reduced graphene oxide (ERGO). The GO was covalently modified on glassy carbon electrode (GCE), and utilized as a directing platform for in-situ synthesis of electroactive PB. Then the GO was electrochemically treated to reduction form to improve the effective surface area and electroactivity of the sensing interface. The fabrication process was characterized by scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX) and atomic force microscopy (AFM). The results showed that the rich oxygen containing groups play a crucial role for the successful synthesis of PB, and the obtained PB layer on the covalently immobilized GO has good stability. Electrochemical sensing assay showed that the modified electrode had tremendous electrocatalytic property for the reduction of HO. The steady-state current response increased linearly with HO concentrations from 5μM to 1mM with a fast response time (less than 3s). The detection limit was estimated to be 0.8μM. When the sensor was applied for determination of HO released from living cells of macrophages, satisfactory results were achieved.
基于普鲁士蓝（PB）和电化学还原氧化石墨烯（ERGO）开发了一种新型电化学非酶过氧化氢（HO）传感器。氧化石墨烯（GO）被共价修饰在玻碳电极（GCE）上，并用作原位合成电活性PB的导向平台。然后对GO进行电化学处理以还原成还原形式，以提高传感界面的有效表面积和电活性。通过扫描电子显微镜（SEM）、能量色散X射线光谱（EDX）和原子力显微镜（AFM）对制备过程进行了表征。结果表明，富含含氧基团对PB的成功合成起着关键作用，并且在共价固定的GO上获得的PB层具有良好的稳定性。电化学传感分析表明，修饰电极对HO的还原具有巨大的电催化性能。稳态电流响应与HO浓度在5μM至1mM范围内呈线性增加，响应时间快（小于3秒）。检测限估计为0.8μM。当该传感器用于测定巨噬细胞活细胞释放的HO时，取得了满意的结果。
