利用羽叶决明花提取物绿色合成银纳米颗粒的抗胆碱酯酶、抗菌及细胞毒性活性评估
Evaluation of anti-cholinesterase, antibacterial and cytotoxic activities of green synthesized silver nanoparticles using from Millettia pinnata flower extract.
作者信息
Rajakumar Govindasamy, Gomathi Thandapani, Thiruvengadam Muthu, Devi Rajeswari V, Kalpana V N, Chung Ill-Min
机构信息
Department of Applied Bioscience, College of Life and Environmental Science, Konkuk University, Seoul 143 701, South Korea.
Department of Chemistry, D.K.M. College for Women, Vellore, Tamil Nadu, India.
出版信息
Microb Pathog. 2017 Feb;103:123-128. doi: 10.1016/j.micpath.2016.12.019. Epub 2016 Dec 23.
The aim of this study is to develop an easy and eco-friendly method for the synthesis of Ag-NPs using extracts from the medicinal plant, Millettia pinnata flower extract and investigate the effects of Ag-NPs on acetylcholinesterase (AChE), butyrylcholinesterase (BChE), antibacterial and cytotoxicity activity. UV-Vis peak at 438 nm confirmed the Ag-NPs absorbance. The SEM analysis results confirmed the presence of spherical shaped Ag-NPs by a huge disparity in the particle size distribution with an average size of 49 ± 0.9 nm. TEM images revealed the formation of Ag-NPs with spherical shape and sizes in the range between 16 and 38 nm. The Ag-NPs showed an excellent inhibitory efficacy against AChE and BChE. The highest antibacterial activity was found against Escherichia coli (20.25 ± 0.91 mm). These nanoparticles showed the cytotoxic effects against brine shrimp (artemia saliana) nauplii with a LD value of 33.92.
本研究的目的是开发一种简便且环保的方法，利用药用植物翅荚决明花提取物合成银纳米颗粒，并研究银纳米颗粒对乙酰胆碱酯酶（AChE）、丁酰胆碱酯酶（BChE）、抗菌和细胞毒性活性的影响。438nm处的紫外可见峰证实了银纳米颗粒的吸光度。扫描电子显微镜（SEM）分析结果通过粒径分布的巨大差异证实了球形银纳米颗粒的存在，平均粒径为49±0.9nm。透射电子显微镜（TEM）图像显示形成了球形且尺寸在16至38nm之间的银纳米颗粒。银纳米颗粒对AChE和BChE表现出优异的抑制效果。对大肠杆菌的抗菌活性最高（20.25±0.91mm）。这些纳米颗粒对卤虫无节幼体表现出细胞毒性作用，半数致死剂量（LD）值为33.92。
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