Dianzani F, Capobianchi M R, Antonelli G, Amicucci P, De Marco F
Institute of Virology, University of Rome, Italy.
Antiviral Res. 1989 Jun-Jul;11(5-6):299-305. doi: 10.1016/0166-3542(89)90039-9.
Under single growth cycle conditions in C8166 lymphoblastoid cells human immunodeficiency virus shows a replication curve which is completed at 24 h post-infection. At lower multiplicity of infection virus yield peaks at approximately 72 h post-infection but in both cases the titer of the virus released in the medium is negligible with respect to that which remains cell-associated. A method based on back-titration of virus in cryolysates of C8166 cells infected with HIV and treated with antiviral compounds has been used to evaluate HIV sensitivity to such agents. Under single growth cycle conditions dose response curves appear linear and permit rapid and accurate determination of the endpoint activity. Under multiple growth cycle conditions the inhibitory activity may be measured during the exponential growth phase, at 48 h post-infection. This method, which directly measures production of infectious virus rather than indirect probes of viral replication such as reverse transcriptase or antigen production, offers the advantage of a precise determination of the degree of activity of antivirals also acting on viral assembly or release.
在C8166淋巴母细胞样细胞的单生长周期条件下，人类免疫缺陷病毒呈现出一条在感染后24小时完成的复制曲线。在较低的感染复数下，病毒产量在感染后约72小时达到峰值，但在这两种情况下，相对于仍与细胞相关的病毒，培养基中释放的病毒滴度可忽略不计。一种基于对感染HIV并用抗病毒化合物处理的C8166细胞冷冻裂解物中的病毒进行反向滴定的方法，已被用于评估HIV对此类药物的敏感性。在单生长周期条件下，剂量反应曲线呈线性，可快速准确地确定终点活性。在多个生长周期条件下，抑制活性可在感染后48小时的指数生长期进行测量。这种方法直接测量传染性病毒的产生，而不是像逆转录酶或抗原产生等病毒复制的间接指标，具有精确确定也作用于病毒组装或释放的抗病毒药物活性程度的优点。
