Institute of Virology, V. le di Porta Tiburtina 28, 00185, Rome, Italy.
Cytotechnology. 1991 Feb;5(Suppl 1):3-6. doi: 10.1007/BF00736794.
Replication Kinetics of HIV was studied under single-growth cycle conditions in C8166 lymphoblastoid cells by back-titrating released and cell-associated infectious virus et each time point. Under these conditions HIV seemed to replicate faster than previously estimated. The amount cell-associated virus always exceeded that detectable in the medium. In these conditions the replication curve of HIV is completed at 24 h post infection. At lower multiplicity of infection, virus yield peaks at approximately 72 h post-infection, and the virus released in the medium is negligible with respect to that which remains cell-associated. The method based on back-titration of virus in cryolysates of C8166 cells infected with HIV and treated with antiviral compounds has been used to evaluate HIV sensitivity to some agents. This method allows a precise and quick determination of the degree of activity of antiviral drugs.
在 C8166 淋巴母细胞系中，通过每次在时间点回滴定释放的和细胞相关的感染性病毒，在单个生长周期条件下研究 HIV 的复制动力学。在这些条件下，HIV 的复制似乎比以前估计的要快。细胞相关病毒的数量总是超过可在培养基中检测到的数量。在这些条件下，HIV 的复制曲线在感染后 24 小时完成。在较低的感染复数下，病毒产量在感染后约 72 小时达到峰值，而释放到培养基中的病毒相对于与细胞相关的病毒可以忽略不计。该方法基于用抗病毒化合物处理感染 HIV 的 C8166 细胞的冷冻裂解物中病毒的回滴定，已用于评估 HIV 对某些药物的敏感性。该方法允许精确和快速确定抗病毒药物的活性程度。
