Liu Yonghua, Wu Weijie, Yang Huiguang, Zhou Zhengming, Zhu Xiaojian, Sun Chi, Liu Yuxi, Yu Zhaohui, Chen Yuyan, Wang Youhua
Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, 19 Qixiu Road, Nantong, Jiangsu, 226001, People's Republic of China.
Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong University, Nantong, Jiangsu, 226001, People's Republic of China.
Neurochem Res. 2017 Apr;42(4):1084-1095. doi: 10.1007/s11064-016-2142-3. Epub 2016 Dec 26.
Tripartite motif containing 32 (TRIM32), a member of the tripartite motif (TRIM) family, plays an indispensable role in myoblast proliferation. It also regulates neuron and skeletal muscle stem cell differentiation. Although it is of great importance, we know little about the roles of TRIM32 during peripheral nervous system injury. Here, we examined the dynamic changes of TRIM32 in acute sciatic nerve crush (SNC) model. After crush, TRIM32 rapidly increased and reached the climax at 1 week but then gradually declined to the normal level at 4 weeks post-injury. Meanwhile, we observed similar changes of Oct-6. What is more, we found co-localization of TRIM32 with S100 and Oct-6 in 1-week-injured tissues using double immunofluorescent staining. In further vitro experiments, enhancive expression of TRIM32 was detected during the process of cyclic adenosine monophosphate (cAMP)-induced Schwann cell differentiation and nerve growth factor (NGF)-induced PC12 cell neurite outgrowth. More interestingly, specific si-TRIM32-transfected RSC96 cells exhibited obvious reduction in the ability of migration. Taken together, we inferred that upregulated TRIM32 was not only involved in the differentiation and migration of Schwann cells but the neurite elongation after SNC.
含三联基序蛋白32（TRIM32）是三联基序（TRIM）家族的成员之一，在成肌细胞增殖中发挥着不可或缺的作用。它还调节神经元和骨骼肌干细胞的分化。尽管其非常重要，但我们对TRIM32在周围神经系统损伤中的作用了解甚少。在此，我们在急性坐骨神经挤压（SNC）模型中检测了TRIM32的动态变化。挤压后，TRIM32迅速增加并在1周时达到高峰，但随后在损伤后4周逐渐下降至正常水平。同时，我们观察到Oct-6有类似变化。此外，我们通过双重免疫荧光染色发现在损伤1周的组织中TRIM32与S100和Oct-6共定位。在进一步的体外实验中，在环磷酸腺苷（cAMP）诱导的雪旺细胞分化和神经生长因子（NGF）诱导的PC12细胞神经突生长过程中检测到TRIM32表达增强。更有趣的是，特异性转染si-TRIM32的RSC96细胞迁移能力明显降低。综上所述，我们推断上调的TRIM32不仅参与雪旺细胞的分化和迁移，还参与坐骨神经挤压后的神经突伸长。
