Su Liling, Wei Xiaoxia, Xu Zhengping, Chen Guangdi
Bioelectromagnetics Laboratory, Zhejiang University School of Medicine, Hangzhou, China.
Department of Public Health, Zhejiang University School of Medicine, Hangzhou, China.
Bioelectromagnetics. 2017 Apr;38(3):175-185. doi: 10.1002/bem.22032. Epub 2016 Dec 27.
Despite many years of studies, the debate on genotoxic effects of radiofrequency electromagnetic fields (RF-EMF) continues. To systematically evaluate genotoxicity of RF-EMF, this study examined effects of RF-EMF on DNA damage and cellular behavior in different neurogenic cells. Neurogenic A172, U251, and SH-SY5Y cells were intermittently (5 min on/10 min off) exposed to 1800 MHz RF-EMF at an average specific absorption rate (SAR) of 4.0 W/kg for 1, 6, or 24 h. DNA damage was evaluated by quantification of γH2AX foci, an early marker of DNA double-strand breaks. Cell cycle progression, cell proliferation, and cell viability were examined by flow cytometry, hemocytometer, and cell counting kit-8 assay, respectively. Results showed that exposure to RF-EMF at an SAR of 4.0 W/kg neither significantly induced γH2AX foci formation in A172, U251, or SH-SY5Y cells, nor resulted in abnormal cell cycle progression, cell proliferation, or cell viability. Furthermore, prolonged incubation of these cells for up to 48 h after exposure did not significantly affect cellular behavior. Our data suggest that 1800 MHz RF-EMF exposure at 4.0 W/kg is unlikely to elicit DNA damage or abnormal cellular behaviors in neurogenic cells. Bioelectromagnetics. 38:175-185, 2017. © 2016 Wiley Periodicals, Inc.
尽管经过多年研究，但关于射频电磁场（RF - EMF）遗传毒性效应的争论仍在继续。为了系统评估RF - EMF的遗传毒性，本研究检测了RF - EMF对不同神经源性细胞中DNA损伤和细胞行为的影响。将神经源性A172、U251和SH - SY5Y细胞间歇性地（开5分钟/关10分钟）暴露于平均比吸收率（SAR）为4.0 W/kg的1800 MHz RF - EMF下1、6或24小时。通过定量γH2AX病灶（DNA双链断裂的早期标志物）来评估DNA损伤。分别通过流式细胞术、血细胞计数器和细胞计数试剂盒 - 8检测法检测细胞周期进程、细胞增殖和细胞活力。结果表明，暴露于SAR为4.0 W/kg的RF - EMF既未在A172、U251或SH - SY5Y细胞中显著诱导γH2AX病灶形成，也未导致细胞周期进程、细胞增殖或细胞活力异常。此外，这些细胞在暴露后长达48小时的延长孵育并未显著影响细胞行为。我们的数据表明，暴露于4.0 W/kg的1800 MHz RF - EMF不太可能在神经源性细胞中引发DNA损伤或异常细胞行为。《生物电磁学》。38:175 - 185，2017年。© 2016威利期刊公司。
