Department of Chemistry, Fudan University , Shanghai 200433, P. R. China.
ACS Appl Mater Interfaces. 2017 Jan 25;9(3):2052-2058. doi: 10.1021/acsami.6b12047. Epub 2017 Jan 11.
The monitoring of reactive oxygen species (ROS) in living cells remains challenging because of the complexity, short half-life, and autofluorescence of biological samples. In this work, we designed a ratiometric fluorescent probe for the detection and imaging of ROS, which was constructed from silicon quantum dots (Si QDs) with chlorin e6 (Ce6) through electrostatic attraction and showed well-resolved dual fluorescence emission signals (490 and 660 nm). Sensitive and selective biosensing of hydroxyl radical (OH) was demonstrated on the basis of fluorescence quenching of the Si QDs and Ce6 as an internal reference to avoid environmental interference, with a detection limit of ∼0.97 μM. The endogenous release of OH was also monitored and imaged in living cells.
在活细胞中监测活性氧（ROS）仍然具有挑战性，因为生物样本的复杂性、短半衰期和自体荧光。在这项工作中，我们设计了一种比率荧光探针用于 ROS 的检测和成像，它是由硅量子点（Si QDs）通过静电吸引与叶绿素 e6（Ce6）构建而成，显示出分辨良好的双荧光发射信号（490nm 和 660nm）。基于 Si QDs 和 Ce6 的荧光猝灭以及作为内部参考以避免环境干扰的羟基（OH）的灵敏和选择性生物传感，检测限约为 0.97μM。还在活细胞中监测和成像了内源性 OH 的释放。
