Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of the Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710127, PR China.
College of Life Sciences, Northwest University, Xi'an 710069, PR China.
Talanta. 2019 May 1;196:317-324. doi: 10.1016/j.talanta.2018.12.065. Epub 2018 Dec 22.
A new ratiometric fluorescent probe (4) for monitoring hydroxyl radicals (•OH) has been designed and synthesized. The ratiometric sensing of probe 4 toward •OH is realized by the oxidation of the dicyanovinyl group of probe 4 to afford the corresponding epoxide, which can further reaction with •OH to give a coumarin derivative as the final product. The above reaction interrupts the large π-conjugated system of probe 4 and exhibits a remarkable large blue shift of 160 nm in the emission spectra of the sensing system. Probe 4 exhibits high selectivity toward •OH over other reactive oxygen species and reactive nitrogen species. Preliminary study shows that probe 4 can be employed to monitor endogenous •OH in living cells with a ratiometric fluorescent imaging manner.
一种用于监测羟基自由基（•OH）的新型比率荧光探针（4）已经被设计和合成。探针 4 对•OH 的比率感应是通过探针 4 的二氰基乙烯基基团的氧化来实现的，这会产生相应的环氧化物，它可以与•OH 进一步反应，生成作为最终产物的香豆素衍生物。上述反应中断了探针 4 的大π共轭体系，并在传感体系的发射光谱中显示出显著的 160nm 大蓝移。探针 4 对•OH 具有很高的选择性，超过了其他活性氧和活性氮物质。初步研究表明，探针 4 可以用于以比率荧光成像方式监测活细胞内的内源性•OH。
