Tang Xiao-Yue, Liu Yi-Ming, Bai Xiao-Lin, Yuan Hao, Hu Yi-Kao, Yu Xiao-Ping, Liao Xun
Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
Department of Chemistry and Biochemistry, Jackson State University, 1400 Lynch St., Jackson, MS, 39217, USA.
Anal Chim Acta. 2021 May 1;1157:338394. doi: 10.1016/j.aca.2021.338394. Epub 2021 Mar 13.
Dopamine (DA) is a critical biomarker for a variety of neurological diseases. Methods for simple and rapid DA detection are crucial for clinical diagnosis and treatments for those diseases. In this work, we developed a novel pretreatment-free method for dopamine detection using carbon dots as a turn-on fluorescent probe synthesized in situ. The aminosilane-functionalized carbon dots (SiCDs) were produced in a mild condensation reaction between N-[3-(Trimethoxysilyl)propyl]ethylenediamine (AEATMS) and dopamine, which were directly used for probing of dopamine. The prepared SiCDs exhibited green fluorescence with excitation/emission maximum at 380/495 nm, the intensity of which can be measured to quantify the DA present in the reaction mixture. The linear range of the assay was between 0.1 and 100 μM with a limit of detection (LOD) of 56.2 nM. The probe is of good selectivity and the recoveries of the developed method were in the range of 101.77-119.91% with RSDs within 3.67% in human serum sample tests. The SiCDs were also synthesized within MN9D cells under 37 °C and generated bright fluorescence, which can probe the DA's distribution in the cells. The described method exhibit potential in DA detection and live-cell imaging for its feature of facility, inexpensiveness, and sensitivity.
多巴胺（DA）是多种神经疾病的关键生物标志物。简单快速检测多巴胺的方法对于这些疾病的临床诊断和治疗至关重要。在这项工作中，我们开发了一种新型的无需预处理的多巴胺检测方法，使用原位合成的碳点作为开启型荧光探针。氨基硅烷功能化碳点（SiCDs）是在N-[3-(三甲氧基硅基)丙基]乙二胺（AEATMS）与多巴胺的温和缩合反应中产生的，可直接用于探测多巴胺。制备的SiCDs在380/495nm处呈现激发/发射最大值的绿色荧光，其强度可用于量化反应混合物中存在的多巴胺。该检测方法的线性范围为0.1至100μM，检测限（LOD）为56.2 nM。该探针具有良好的选择性，在人血清样本测试中，所开发方法的回收率在101.77-119.91%范围内，相对标准偏差（RSD）在3.67%以内。SiCDs也在37°C下在MN9D细胞内合成并产生明亮荧光，可探测多巴胺在细胞内的分布。所描述的方法因其简便、廉价和灵敏的特点，在多巴胺检测和活细胞成像方面具有潜力。
