Fujita M, Tanigawa K, Fujita F, Sakamoto Y, Shimozuma K, Kusuyama T, Inaba H, Taguchi T
Dept. of Surgery, Osaka University.
Gan To Kagaku Ryoho. 1989 Oct;16(10):3435-41.
Twenty lines of human gastro intestinal and breast cancer xenografts, in which chemosensitivity spectra by the in vivo nude mouse assay had been clarified. were subjected to the in vitro SDI (succinate dehydrogenase inhibition) assay using MTT dye to assess the accuracy of this drug sensitivity test against 4 drugs i.e., mitomycin C (MMC), adriamycin (ADM) 5 fluorouracil (5-FU), and cisplatin (CDDP). After 3 days incubation, the suspension of every tumor cells including small fragments showed a marked decrease of SD activity even when no anticancer drug was added to the assay medium. Among these 4 drugs evaluated MMC exhibited a statistically significant correlation between chemosensitivity values of the in vitro SDI assay and those of the nude mouse assay. However, the other 3 drugs demonstrated no correlation between the values of these two methods. Since the primary cultured fibroblasts revealed, in general, lower sensitivity to these drugs, contamination of fibroblast may decrease the SDI values when materials from solid tumors with rich stroma such as a type of stomach cancer were subjected. It is considered that the prediction of chemosensitivity to every drug will be impossible by a in vitro SDI assay.
20株人胃肠道和乳腺癌异种移植瘤，其体内裸鼠试验的化学敏感性谱已明确。使用MTT染料对其进行体外SDI（琥珀酸脱氢酶抑制）试验，以评估该药物敏感性试验对丝裂霉素C（MMC）、阿霉素（ADM）、5-氟尿嘧啶（5-FU）和顺铂（CDDP）这4种药物的准确性。培养3天后，即使在试验培养基中未添加抗癌药物，包括小碎片在内的每个肿瘤细胞悬液的SD活性也显著降低。在评估的这4种药物中，MMC在体外SDI试验和裸鼠试验的化学敏感性值之间表现出统计学上的显著相关性。然而，其他3种药物在这两种方法的值之间未显示相关性。由于原代培养成纤维细胞通常对这些药物敏感性较低，当使用来自富含基质的实体瘤（如一种胃癌）的材料时，成纤维细胞的污染可能会降低SDI值。认为通过体外SDI试验不可能预测每种药物的化学敏感性。
