Department of Bioengineering, University of Washington, Seattle, WA 98195, United States.
Nanotechnology. 2017 Feb 17;28(7):075103. doi: 10.1088/1361-6528/aa55e0. Epub 2016 Dec 28.
We report a simple and versatile method for in vitro fabrication of scaffold-free tissue-engineered constructs with predetermined cellular alignment, by combining magnetic cell levitation with thermoresponsive nanofabricated substratum (TNFS) based cell sheet engineering technique. The TNFS based nanotopography provides contact guidance cues for regulation of cellular alignment and enables cell sheet transfer, while magnetic nanoparticles facilitate the magnetic levitation of the cell sheet. The temperature-mediated change in surface wettability of the thermoresponsive poly(N-isopropylacrylamide), substratum enables the spontaneous detachment of cell monolayers, which can then be easily manipulated through use of a ring or disk shaped magnet. Our developed platform could be readily applicable to production of tissue-engineered constructs containing complex physiological structures for the study of tissue structure-function relationships, drug screening, and regenerative medicine.
我们报告了一种简单而通用的方法，用于通过结合磁细胞悬浮和基于热响应纳米制造基底（TNFS）的细胞片工程技术，在体外制造具有预定细胞取向的无支架组织工程构建体。基于 TNFS 的纳米形貌为调节细胞取向提供了接触引导线索，并能够进行细胞片转移，而磁性纳米颗粒则有助于细胞片的磁悬浮。温敏聚（N-异丙基丙烯酰胺）基底的表面润湿性的温度介导变化可实现细胞单层的自发脱落，然后可以通过使用环形或盘形磁铁轻松地对其进行操作。我们开发的平台可以很容易地应用于生产包含复杂生理结构的组织工程构建体，用于研究组织结构-功能关系、药物筛选和再生医学。
