Photodynamic Inactivation of E. coli PTCC 1276 Using Light Emitting Diodes: Application of Rose Bengal and Methylene Blue as Two Simple Models.

The lack of a comparative study about potential of high-power light emitting diodes (LEDs) for photodynamic inactivation (PDI) of pathogenic microorganisms has remained as a challenging issue for researchers. Therefore, the aim of this study is to fill this gap through introduction of an efficient model for in vitro PDI in an aqueous medium. For this purpose, two individual 30 mW/cm irradiation systems were designed using suitable sets of green and red LEDs. At another work, Methylene blue (MB) and Rose bengal (RB) as two simple models in the range of 5-150 μM were used in order to compare PDI of E. coli PTCC 1276 using red and green LED systems. Our results showed that a first-order mathematical model has the strength to describe the temporal variation of survival curves. Based on our results, when concentration of photosensitizer increased, the rate of inactivation for RB increased while MB depicted a maximum rate value at 25 μM. In a comparative study, optimum inactivation of E. coli PTCC 1276 obtained during 2- and 10-min irradiation of the LED systems using RB and MB at 150 and 25 μM, respectively. With regard to lower value of inactivation time and higher rate of inactivation for RB, use of simultaneous green high-power LEDs and RB is proposed as an efficient approach for PDI of pathogenic bacteria in future industrial applications.