Department of Microbiology, Hospital Universitario Miguel Servet, Zaragoza, Spain; University of Zaragoza, Zaragoza, Spain.
Department of Microbiology, Hospital Universitario Miguel Servet, Zaragoza, Spain.
Photodiagnosis Photodyn Ther. 2017 Sep;19:98-102. doi: 10.1016/j.pdpdt.2017.05.017. Epub 2017 May 22.
Antimicrobial photodynamic therapy (aPDT) has shown to exert a bactericidal effect against Streptococcus sanguinis and Streptococcus mutans. However, this efficacy has been reported for either type of bacteria separately. Bacterial suspensions of both strains, separately or together, were treated with concentrations of methylene blue (MB) and rose bengal (RB). Suspensions were irradiated with a light-emitting diode lamp (λ center at 625nm for MB and λ center at 515nm for RB) using a fluence of 18J/cm. RB-aPDT at concentrations of 0.16-0.62 and 0.16-0.31μg/mL, and MB-aPDT at concentrations of 0.62-1.25 and 0.31-1.25μg/mL inhibited the growth of S. mutans and S. sanguinis respectively by 6 log. In suspensions of both strains together, the same 6 log reduction in bacterial growth was achieved using the same concentrations of each photosensiziser. In conclusion, RB-aPDT and MB-aPDT appear to exert the same bactericidal effect against suspensions of S. sanguinis and S. mutans either for single strain treatment or for samples constituted by both bacteria mixed together. RB shows to be slightly more efficient than MB.
抗菌光动力疗法（aPDT）已被证明对血链球菌和变形链球菌具有杀菌作用。然而，这种功效仅针对这两种细菌中的一种进行了报道。将两种菌株的细菌悬浮液分别或混合在一起，用亚甲蓝（MB）和孟加拉玫瑰红（RB）的浓度处理。用发光二极管灯（MB 的λ中心在 625nm，RB 的λ中心在 515nm）以 18J/cm 的剂量照射悬浮液。RB-aPDT 的浓度为 0.16-0.62 和 0.16-0.31μg/mL，MB-aPDT 的浓度为 0.62-1.25 和 0.31-1.25μg/mL，分别抑制了 S. mutans 和 S. sanguinis 的生长 6 对数。在两种菌株的混合悬浮液中，使用相同浓度的每种光敏剂，可实现相同的 6 对数细菌生长减少。综上所述，RB-aPDT 和 MB-aPDT 似乎对单独处理或由两种细菌混合组成的样本中的 S. sanguinis 和 S. mutans 悬浮液具有相同的杀菌作用。RB 比 MB 略有效。
