VIP Clinic and Multi-Disciplinary Treatment Center, Beijing Stomatological Hospital & School of Stomotology, Capital Medical University, Beijing, China.
Department of Periodontology, Beijing Stomatological Hospital & School of Stomotology, Capital Medical University, Beijing, China.
Photodiagnosis Photodyn Ther. 2021 Jun;34:102250. doi: 10.1016/j.pdpdt.2021.102250. Epub 2021 Mar 9.
The main goal of periodontal therapy is to eliminate the spread of infection in the periodontium. Antimicrobial photodynamic therapy (aPDT) is a bactericidal method that has been recently introduced for controlling periodontal infection. The aim of this in vitro study was to evaluate the effect of aPDT using a combination of medium-power blue light-emitting diodes (LEDs) and rose bengal (RB) on selected key periodontopathogens.
Porphyromonas gingivalis ATCC33277, Aggregatibacter actinomycetemcomitans ATCC29523 and Fusobacterium nucleatum ATCC10953 were used in the experiments. Each bacterial suspension was irradiated with a blue LED (BL) (450-470 nm, output power density of 1.2 W/cm) for 20-60 s (6-18 J/cm), treated with RB (1 min), or subjected to a combination of RB treatment and BL irradiation (40 s, 12 J/cm). All bacterial suspensions were serially diluted, plated and incubated anaerobically or microaerobically, and the numbers of colony-forming units (CFUs) were counted on day 7. One-way analysis of variance (ANOVA) and Tukey's HSD tests were used for statistical analysis.
Treatment with BL irradiation from 6 to 18 J/cm did not significantly reduce the number of CFUs, whereas treatment with RB alone induced a low-to-high reduction in the bacterial CFUs in a dye concentration-dependent manner. Furthermore, the difference in the effects obtained with 16 μg/mL and 160 μg/mL RB was not statistically significant. Treatment with the BL at 12 J/cm combined with 160 μg/mL RB yielded maximal log reductions of 3.03, 4.2 and 2.23 in P. gingivalis, A. actinomycetemcomitans and F. nucleatum CFUs, respectively.
Within the limits of this study, the three periodontal pathogens, especially A. actinomycetemcomitans, were susceptible to photodynamic inactivation by the combination of the BL and RB. RB-mediated aPDT may offer a viable alternative tool for periodontal pathogen treatment, especially for A. actinomycetemcomitans eradication. aPDT may be a valuable tool for the treatment of periodontal diseases, particularly those in which A. actinomycetemcomitans is a dominating pathogen.
牙周病治疗的主要目标是消除牙周组织中的感染扩散。 抗菌光动力疗法（aPDT）是一种最近引入的控制牙周感染的杀菌方法。 本体外研究的目的是评估使用中功率蓝光发光二极管（LED）和玫瑰红（RB）组合对选定关键牙周病原体的 aPDT 效果。
实验中使用牙龈卟啉单胞菌 ATCC33277、伴放线放线杆菌 ATCC29523 和核梭杆菌 ATCC10953。 每个细菌悬浮液用蓝色 LED（BL）（450-470nm，输出功率密度为 1.2W/cm）照射 20-60s（6-18J/cm），用 RB 处理（1 分钟），或用 RB 处理和 BL 照射组合（40s，12J/cm）。 所有细菌悬浮液均进行连续稀释、平板接种和厌氧或微需氧孵育，并在第 7 天计数集落形成单位（CFU）的数量。 采用单因素方差分析（ANOVA）和 Tukey 的 HSD 检验进行统计分析。
6 至 18J/cm 的 BL 照射处理不会显著减少 CFU 数量，而单独使用 RB 处理则以染料浓度依赖性方式导致细菌 CFU 低至高减少。 此外，用 16μg/mL 和 160μg/mL RB 获得的效果差异在统计学上无显著性。 用 12J/cm 的 BL 与 160μg/mL RB 联合处理可使牙龈卟啉单胞菌、伴放线放线杆菌和核梭杆菌 CFU 的最大对数减少分别为 3.03、4.2 和 2.23。
在本研究范围内，三种牙周病原体，特别是伴放线放线杆菌，对 BL 和 RB 联合的光动力失活敏感。 RB 介导的 aPDT 可能为牙周病病原体治疗提供一种可行的替代工具，特别是用于根除伴放线放线杆菌。 aPDT 可能是治疗牙周病的有价值工具，特别是在伴放线放线杆菌是主要病原体的情况下。
