Skucha-Nowak Małgorzata, Machorowska-Pieniążek Agnieszka, Tanasiewicz Marta
Department of Conservative Dentistry with Endodontics, Medical University of Silesia in Katowice, Bytom, Poland.
Department of Orthodontics, School of Medicine with Division of Dentistry in Zabrze, Medical University of Silesia in Katowice, Poland.
Adv Clin Exp Med. 2016 Sep-Oct;25(5):961-969. doi: 10.17219/acem/63007.
The aim of the infiltration technique is to penetrate demineralized enamel with a low viscosity resin. Icon® (DMG) is the first ever and so far the only dental infiltrant. Bacteriostaticity is one of the properties that should be inherent in dental infiltrants, but Icon lacks this feature.
The aim of the preliminary study was to properly choose a dye which would allow us to assess the penetrating abilities of our own, experimental preparation with features of a dental infiltrant with bacteriostatic properties and to compare using an optical microscope the depth of infiltration of the designed experimental preparation with the infiltrant available on the market.
The preparation is supposed to infiltrate decalcified human enamel and be assessed with an optical microscope. Eosin, neutral fuchsine and methylene blue were added to experimental preparation with dental infiltrant features and to Icon® (DMG) in order to assess the depth of penetration of the experimental solution into the decalcified layers of enamel.
The experimental solution mixes well with eosin, neutral fuchsine, and methylene blue.
During the preliminary study, the authors concluded that the experimental solution mixes well with methylene blue, neutral fuchsine, and eosin. An addition of eosin to a preparation which infiltrates inner, demineralized enamel layers, facilitates the assessment of such a preparation with an optical microscope. A designed experimental solution with the main ingredients, i.e., 2-hydroxyethyl methacrylate (HEMA) and tetraethylene glycol dimethacrylate (TEGDMA) with a ratio of 75% to 25% penetrates the demineralized (decalcified) inner parts of the enamel and polymerizes when exposed to light. In order to assess the infiltration of the experimental solution into the demineralized enamel layers, it is required to improve the measurement techniques that utilize optical microscopy.
渗透技术的目的是用低粘度树脂渗透脱矿釉质。Icon®（DMG）是有史以来第一款也是迄今为止唯一的牙科渗透剂。抑菌性是牙科渗透剂应具备的特性之一，但Icon缺乏这一特性。
初步研究的目的是正确选择一种染料，以便我们评估具有抑菌特性的自制实验制剂作为牙科渗透剂的渗透能力，并使用光学显微镜比较设计的实验制剂与市售渗透剂的渗透深度。
该制剂应渗透脱钙的人牙釉质，并通过光学显微镜进行评估。将曙红、中性品红和亚甲蓝添加到具有牙科渗透剂特性的实验制剂和Icon®（DMG）中，以评估实验溶液渗透到釉质脱钙层中的深度。
实验溶液与曙红、中性品红和亚甲蓝混合良好。
在初步研究中，作者得出结论，实验溶液与亚甲蓝、中性品红和曙红混合良好。向渗透到内部脱矿釉质层的制剂中添加曙红，便于用光学显微镜评估该制剂。一种设计的实验溶液，其主要成分甲基丙烯酸2-羟乙酯（HEMA）和四甘醇二甲基丙烯酸酯（TEGDMA）的比例为75%至25%，可渗透到釉质脱矿（脱钙）的内部，并在光照下聚合。为了评估实验溶液在脱矿釉质层中的渗透情况，需要改进利用光学显微镜的测量技术。
