Skucha-Nowak Małgorzata, Mertas Anna, Tanasiewicz Marta
Department of Conservative Dentistry with Endodontics, Medical University of Silesia in Katowice, Bytom, Poland.
Chair and Department of Microbiology and Immunology, School of Medicine with the Division of Dentistry, Medical University of Silesia in Katowice, Zabrze, Poland.
Adv Clin Exp Med. 2016 Nov-Dec;25(6):1293-1301. doi: 10.17219/acem/63795.
The resin infiltration technique is one of the micro-invasive methods whose aim is the penetration of demineralized enamel with a low viscosity resin. This technique allows the dentist to avoid the application of mechanical means of treatment.
The objective of this preliminary study was to attempt to determine the possibilities of using an electron microscope to assess the penetrating abilities of an experimental preparation with features of a dental infiltrant and to compare the depth of infiltration of the designed experimental preparation with an infiltrant available on the market.
A bioactive methacrylate monomer based on PMMAn with built-in metronidazole was synthesized. The commercially available Icon solution (with contrast agent YbF3) and the experimental solution were applied to the relevant parts of teeth. The dissected sections along the long tooth axis and polished surfaces were then examined with use of an electron scanning microscope.
The backscattered electron technique gives much better results than the secondary electron method as it makes it possible to localize even very small YbF3 particles.
The authors concluded that the backscattered electron technique gives much better results than the secondary electron method as it makes it possible to localize even very small particles of the contrast agent. In order to prevent blockage of decalcified enamel tissue by ytterbium trifluoride (YbF3) grains, a nanoparticle form of that compound should be used (that is, particles with sizes in the range of 10-9 m).
树脂浸润技术是一种微创方法，其目的是用低粘度树脂渗透脱矿釉质。该技术使牙医能够避免采用机械治疗手段。
本初步研究的目的是尝试确定使用电子显微镜评估具有牙科浸润剂特征的实验制剂渗透能力，并将设计的实验制剂与市场上现有浸润剂的浸润深度进行比较的可能性。
合成了一种基于聚甲基丙烯酸甲酯（PMMAn）并内置甲硝唑的生物活性甲基丙烯酸酯单体。将市售的Icon溶液（含造影剂YbF3）和实验溶液应用于牙齿的相关部位。然后使用电子扫描显微镜检查沿牙齿长轴的解剖切片和抛光表面。
背散射电子技术比二次电子方法的效果要好得多，因为它能够定位即使非常小的YbF3颗粒。
作者得出结论，背散射电子技术比二次电子方法的效果要好得多，因为它能够定位即使非常小的造影剂颗粒。为了防止三氟化镱（YbF3）颗粒堵塞脱钙釉质组织，应使用该化合物的纳米颗粒形式（即尺寸在10-9米范围内的颗粒）。
