Skucha-Nowak Małgorzata
Department of Conservative Dentistry with Endodontics, Medical University of Silesia in Katowice, Plac Akademicki 17, 41-902 Bytom, phone: +48 32 282 79 42, fax:+48322827942.
Open Med (Wars). 2015 Apr 3;10(1):238-248. doi: 10.1515/med-2015-0036. eCollection 2015.
The resin infiltration technique, a minimally invasive method, involves the saturation, strengthening, and stabilization of demineralized enamel by a mixture of polymer resins without the need to use rotary tools or the risk of losing healthy tooth structures.
To design and synthesize an experimental infiltrant with potential bacteriostatic properties.To compare the depth of infiltration of the designed experimental preparation with the infiltrant available in the market using a scanning electron microscope.
Composition of the experimental infiltrant was established after analysis of 1H NMR spectra of the commercially available compounds that can penetrate pores of demineralized enamel. As the infiltrant should have bacteriostatic features by definition, an addition of 1% of monomer containing metronidazole was made. Thirty extracted human teeth were soaked in an acidic solution, which was to provide appropriate conditions for demineralization of enamel. Afterward, each tooth was divided along the coronal-root axis into two zones. One zone had experimental preparation applied to it (the test group), while the other had commercially available Icon (the control group). The teeth were dissected along the long axis and described above underwent initial observation with use of a Hitachi S-4200 scanning electron microscope.
It was found that all samples contained only oxygen and carbon, regardless of the concentration of additions introduced into them. The occurrence of carbon is partially because it is a component of the preparation in question and partially because of sputtering of the sample with it. Hydrogen is also a component of the preparation, as a result of its phase composition; however, it cannot be detected by the EDS method.
SEM, in combination with X-ray microanalysis, does not allow one to explicitly assess the depth of penetration of infiltration preparations into enamel.In order to assess the depth of penetration of infiltration preparations with use of X-ray microanalysis, it is recommended to introduce a contrast agent that is approved for use in dental materials, such as ytterbium III fluoride.
树脂渗透技术是一种微创方法，通过聚合物树脂混合物使脱矿釉质饱和、强化和稳定，无需使用旋转工具，也不存在损失健康牙体结构的风险。
设计并合成一种具有潜在抑菌特性的实验性渗透剂。使用扫描电子显微镜比较设计的实验制剂与市售渗透剂的渗透深度。
通过对可渗透脱矿釉质孔隙的市售化合物的1H NMR光谱分析确定实验性渗透剂的成分。由于根据定义渗透剂应具有抑菌特性，添加了1%含甲硝唑的单体。将30颗拔除的人牙浸泡在酸性溶液中，以提供釉质脱矿的适当条件。之后，每颗牙沿冠根轴分为两个区域。一个区域应用实验制剂（试验组），另一个区域应用市售的Icon（对照组）。沿长轴将牙齿切开，使用日立S-4200扫描电子显微镜对上述样本进行初步观察。
发现所有样本仅含有氧和碳，无论引入其中的添加物浓度如何。碳的存在部分是因为它是所讨论制剂的成分，部分是因为样本被其溅射。氢也是制剂的成分，由于其相组成；然而，无法通过能谱分析方法检测到。
扫描电子显微镜结合X射线微分析无法明确评估渗透制剂进入釉质的渗透深度。为了使用X射线微分析评估渗透制剂的渗透深度，建议引入一种批准用于牙科材料的造影剂，如氟化镱III。
