Chevagina N N, Izranov V A
Arkh Anat Gistol Embriol. 1989 Jun;96(6):57-60.
By means of mechanotron 6MYIC and perfusion with physiological solution using the "running vesicle", dynamics of functional activity of the popliteal lymph nodes have been studied in 17 non-inbred dogs under conditions of unilateral sympathectomy for 1 week up to 5 months of the experiment. Dependence of the lymph node functional activity on the adrenomediator concentration level in their tissues has been determined by means of the microfluorometry method in the preparations treated after Falck-Hillarp method in E. M. Krokhina modification. Lymph nodes of intact dogs serve as a control. Unilateral sympathectomy of the lymph node is stated to produce an enhancement of the period for its filling and emptying with lymph, resulting from an increased level of the mediator in the fibrillar stroma at the expense of compensatory enhancement of functional activity of the nervous fibers of the contralateral origin. During the remote postoperative period the amplitude of the lymph node capsule fluctuations is stipulated by certain pathomorphological changes, produced by deficiency in concentration of catecholamines in the organ's parenchyma. As to the periodicity of the capsule fluctuations in the intact lymph nodes, it is limited with the cycle of 3-6 min.
借助6MYIC型肌动描记器，并使用“活动囊泡”灌注生理溶液，在17只非近交系犬身上进行了实验，实验时间从1周到5个月不等，研究了单侧交感神经切除术后腘淋巴结功能活动的动态变化。通过微荧光测定法，在经E.M.克罗希娜改良的法尔克-希拉尔普方法处理的制剂中，确定了淋巴结功能活动对其组织中肾上腺介质浓度水平的依赖性。完整犬的淋巴结作为对照。据指出，单侧交感神经切除会导致淋巴结淋巴充盈和排空期延长，这是由于纤维基质中介质水平升高，这是以对侧起源神经纤维功能活动的代偿性增强为代价的。在术后远期，淋巴结包膜波动的幅度由器官实质中儿茶酚胺浓度不足所产生的某些病理形态学变化所决定。至于完整淋巴结包膜波动的周期性，其周期限制在3 - 6分钟。
