Simian Marina, Bissell Mina J
Instituto de Nanosistemas, Universidad Nacional de San Martín, San Martín 1650, Provincia de Buenos Aires, Argentina
Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720
J Cell Biol. 2017 Jan 2;216(1):31-40. doi: 10.1083/jcb.201610056. Epub 2016 Dec 28.
In the last ten years, there has been a dramatic surge in the number of publications where single or groups of cells are grown in substrata that have elements of basement membrane leading to the formation of tissue-like structures referred to as organoids. However, this field of research began many decades ago, when the pioneers of cell culture began to ask questions we still ask today: How does organogenesis occur? How do signals integrate to make such vastly different tissues and organs given that the sequence of the genome in our trillions of cells is identical? Here, we summarize how work over the past century generated the conceptual framework that has allowed us to make progress in the understanding of tissue-specific morphogenetic programs. The development of cell culture systems that provide accurate and physiologically relevant models are proving to be key in establishing appropriate platforms for the development of new therapeutic strategies.
在过去十年中，在含有基底膜成分的基质中培养单个细胞或细胞群以形成被称为类器官的组织样结构的出版物数量急剧增加。然而，这一研究领域始于数十年前，当时细胞培养的先驱们开始提出我们如今仍在探讨的问题：器官发生是如何发生的？鉴于我们数万亿个细胞中的基因组序列是相同的，信号是如何整合以形成如此截然不同的组织和器官的？在此，我们总结了过去一个世纪的研究如何构建起概念框架，使我们在理解组织特异性形态发生程序方面取得进展。事实证明，开发能够提供准确且生理相关模型的细胞培养系统是建立新型治疗策略开发合适平台的关键。
