Sato Ana Claudia, Bosch Rosemary Viola, Will Sonia Elisabete Alves, Alvarez-Flores Miryam Paola, Goldfeder Mauricio Barbugiani, Pasqualoto Kerly Fernanda Mesquita, da Silva Bárbara Athayde Vaz Galvão, de Andrade Sonia Aparecida, Chudzinski-Tavassi Ana Marisa
Laboratory of Biochemistry and Biophysics, Butantan Institute, Av. Vital Brasil 1500, São Paulo, SP 05503-900 Brazil.
J Venom Anim Toxins Incl Trop Dis. 2016 Dec 21;22:36. doi: 10.1186/s40409-016-0093-4. eCollection 2016.
Hemolin proteins are cell adhesion molecules from lepidopterans involved in a wide range of cell interactions concerning their adhesion properties. However, hemolin's roles in cell proliferation and wound healing are not fully elucidated. It has been recently reported that rLosac, a recombinant hemolin from the caterpillar , presents antiapoptotic activity and is capable of improving in vitro wound healing. Therefore, this study aimed to explore rLosac's in vivo effects using a skin wound healing model in rats.
Circular full-thickness wounds in the rat dorsum skin were treated either with rLosac, or with saline (control), allowing healing by keeping the wounds occluded and moist. During the wound healing, the following tissue regeneration parameters were evaluated: wound closure and collagen content. Furthermore, tissue sections were subjected to histological and immunohistochemical analyses.
The rLosac treatment has demonstrated its capacity to improve wound healing, as reflected in findings of a larger number of activated fibroblasts, proliferation of epithelial cells, increase of collagen type 1, and decrease of inflammatory infiltrate.
The findings have indicated the rLosac protein as a very promising molecule for the development of new wound-healing formulations.
血淋巴蛋白是鳞翅目昆虫的细胞粘附分子，参与了一系列与其粘附特性相关的细胞相互作用。然而，血淋巴蛋白在细胞增殖和伤口愈合中的作用尚未完全阐明。最近有报道称，来自毛虫的重组血淋巴蛋白rLosac具有抗凋亡活性，并且能够促进体外伤口愈合。因此，本研究旨在使用大鼠皮肤伤口愈合模型探索rLosac的体内作用。
用rLosac或生理盐水（对照）处理大鼠背部皮肤的圆形全层伤口，通过保持伤口封闭和湿润来促进愈合。在伤口愈合过程中，评估以下组织再生参数：伤口闭合和胶原蛋白含量。此外，对组织切片进行组织学和免疫组织化学分析。
rLosac治疗已证明其具有促进伤口愈合的能力，表现为大量活化的成纤维细胞、上皮细胞增殖、I型胶原蛋白增加以及炎症浸润减少。
研究结果表明，rLosac蛋白是开发新型伤口愈合制剂的非常有前景的分子。
