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D-氨基酸氧化酶-磁性纳米颗粒系统的新合成与生物分布

New synthesis and biodistribution of the D-amino acid oxidase-magnetic nanoparticle system.

作者信息

Cappellini Francesca, Recordati Camilla, Maglie Marcella De, Pollegioni Loredano, Rossi Federica, Daturi Marco, Gornati Rosalba, Bernardini Giovanni

机构信息

Dipartimento di Biotecnologie e Scienze della Vita, Università degli Studi dell'Insubria, Via Dunant 3, Varese, Italy; Dipartimento di Biotecnologie e Scienze della Vita, Università degli Studi dell'Insubria, Via Dunant 3, Varese, Italy.

Mouse & Animal Pathology Laboratory, Fondazione Filarete, Viale Ortles, Milano, Italy; Mouse & Animal Pathology Laboratory, Fondazione Filarete, Viale Ortles, Milano, Italy.

出版信息

Future Sci OA. 2015 Sep 11;1(4):FSO67. doi: 10.4155/fso.15.67. eCollection 2015 Nov.

DOI:10.4155/fso.15.67
PMID:28031918
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5138019/
Abstract

BACKGROUND

Application of nanoenzymes, based on D-amino acid oxidase (DAAO) conjugated to magnetic nanoparticles (NPs), as anticancer system requires improvement of the synthesis protocol and distribution evaluation.

RESULTS

A new and more efficient synthesis via EDC-NHS produced an FeONP-APTES-DAAO system with a specific activity of 7 U/mg NPs. IR spectroscopy showed that all FeO NP sites are saturated with APTES and all available NH sites with DAAO. The acute cytotoxicity of the new system does not differ from that of the previous one. experiments showed that the system did not cause adverse effects, cross the brain-blood barrier and accumulate in the heart.

CONCLUSIONS

Our results support the possibility to use enzymes conjugated to magnetic NPs for cancer treatment. Besides, we think that enzymes and other biological molecules efficiently conjugated to magnetic NPs might constitute a category of 'bionanoparticles' to be exploited, not only in medical, but also in industrial biotechnology.

摘要

背景

基于与磁性纳米颗粒（NPs）偶联的D-氨基酸氧化酶（DAAO）的纳米酶作为抗癌系统的应用需要改进合成方案并进行分布评估。

结果

通过EDC-NHS进行的一种新的、更高效的合成产生了一种比活性为7 U/mg NPs的FeONP-APTES-DAAO系统。红外光谱表明，所有FeO NP位点都被APTES饱和，所有可用的NH位点都被DAAO饱和。新系统的急性细胞毒性与前一个系统没有差异。实验表明，该系统不会引起不良反应，不会穿过血脑屏障，也不会在心脏中蓄积。

结论

我们的结果支持将与磁性NPs偶联的酶用于癌症治疗的可能性。此外，我们认为与磁性NPs有效偶联的酶和其他生物分子可能构成一类有待开发的“生物纳米颗粒”，不仅可用于医学，还可用于工业生物技术。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc9a/5138019/4e201aec5725/fso-01-67-g10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc9a/5138019/c24817d4b20c/fso-01-67-g1.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc9a/5138019/05e754685446/fso-01-67-g5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc9a/5138019/845fbdccb535/fso-01-67-g6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc9a/5138019/6d40561976c7/fso-01-67-g7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc9a/5138019/9ff29af18531/fso-01-67-g8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc9a/5138019/cedf05156984/fso-01-67-g9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc9a/5138019/4e201aec5725/fso-01-67-g10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc9a/5138019/c24817d4b20c/fso-01-67-g1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc9a/5138019/bfda5bbbea62/fso-01-67-g2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc9a/5138019/a0504c9dfadf/fso-01-67-g3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc9a/5138019/a4402902c0ea/fso-01-67-g4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc9a/5138019/05e754685446/fso-01-67-g5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc9a/5138019/845fbdccb535/fso-01-67-g6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc9a/5138019/6d40561976c7/fso-01-67-g7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc9a/5138019/9ff29af18531/fso-01-67-g8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc9a/5138019/cedf05156984/fso-01-67-g9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc9a/5138019/4e201aec5725/fso-01-67-g10.jpg

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2
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J Med Invest. 2015;62(1-2):45-50. doi: 10.2152/jmi.62.45.
3
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3 Biotech. 2023 Jul;13(7):243. doi: 10.1007/s13205-023-03568-1. Epub 2023 Jun 19.
4
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5
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