Sitarek Przemysław, Skała Ewa, Toma Monika, Wielanek Marzena, Szemraj Janusz, Skorski Tomasz, Białas Adam J, Sakowicz Tomasz, Kowalczyk Tomasz, Radek Maciej, Wysokińska Halina, Śliwiński Tomasz
Department of Biology and Pharmaceutical Botany, Medical University of Lodz, Muszyńskiego Street 1, 90-151, Łódź, Poland.
Department of Molecular Genetics, University of Lodz, Łódź, Poland.
Pathol Oncol Res. 2017 Jul;23(3):679-687. doi: 10.1007/s12253-016-0170-6. Epub 2016 Dec 28.
This study determines the influence of transformed root (TR) extract of Leonurus sibiricus L. on various grades (I-III) of human glioma cells derived from patients. This plant occurs in southern Asia and Siberia and is widely used as a medicinal plant with various biological activities. Chromatographic profile of TR extract have revealed the presence of various polyphenolic compounds (4-hydroxybenzoic acid, gentisic acid, vanilic acid, 1,3-dicaffeoylquinic acid, α-resorcylic acid). We found TR root extract to have antiproliferative activity on glioma cells after 24 h of treatment. TR root extract induces apoptosis on various grades (I-III) of human glioma cells by the generation of reactive oxygen species (ROS) along with concurrent loss of mitochondrial membrane potential, enhanced S and G2/M phases of the cell cycle, and altered mRNA levels of Bax, Bcl-2, p53, Cas-3, Cas-8 and Cas-9 factors involved in apoptosis. This work for the first time demonstrate that TR extract from L. sibiricus root has the potential to activate apoptosis in grade I-III human glioma cells through the intrinsic and extrinsic pathways.
本研究确定了益母草转化根提取物对源自患者的不同级别（I - III级）人胶质瘤细胞的影响。这种植物生长在亚洲南部和西伯利亚，作为具有多种生物活性的药用植物被广泛使用。转化根提取物的色谱分析表明存在多种多酚化合物（4 - 羟基苯甲酸、龙胆酸、香草酸、1,3 - 二咖啡酰奎宁酸、α - 间苯二酚）。我们发现处理24小时后，转化根提取物对胶质瘤细胞具有抗增殖活性。转化根提取物通过产生活性氧（ROS）诱导不同级别（I - III级）人胶质瘤细胞凋亡，同时伴有线粒体膜电位丧失、细胞周期S期和G2/M期增强，以及参与凋亡的Bax、Bcl - 2、p53、Cas - 3、Cas - 8和Cas - 9因子的mRNA水平改变。这项工作首次证明，益母草根的转化根提取物有可能通过内源性和外源性途径激活I - III级人胶质瘤细胞的凋亡。
