Sitarek Przemysław, Skała Ewa, Wysokińska Halina, Wielanek Marzena, Szemraj Janusz, Toma Monika, Śliwiński Tomasz
Department of Biology and Pharmaceutical Botany, Medical University of Łódź, Muszyńskiego 1, 90-151 Łódź, Poland.
Department of Plant Physiology and Biochemistry, Faculty of Biology and Environmental Protection, University of Łódź, Banacha 12/16, 90-237 Łódź, Poland.
Oxid Med Cell Longev. 2016;2016:5738193. doi: 10.1155/2016/5738193. Epub 2015 Dec 14.
Leonurus sibiricus L. has been used as a traditional and medicinal herb for many years in Asia and Europe. This species is known to have antibacterial, anti-inflammatory, and antioxidant activity and has demonstrated a reduction of intracellular reactive oxygen species. All tested extracts of L. sibiricus showed protective and DNA repair stimulating effects in Chinese hamster ovary (CHO) cells exposed to H2O2. Preincubation of the CHO cells with 0.5 mg/mL of plant extracts showed increased expression level of antioxidant genes (SOD2, CAT, and GPx). LC-MS/MS and HPLC analyses revealed the presence of nine phenolic compounds in L. sibiricus plant extracts: catechin, verbascoside, two flavonoids (quercetin and rutin), and five phenolic acids (4-hydroxybenzoic acid, chlorogenic acid, caffeic acid, p-coumaric acid, and ferulic acid). The roots and aerial parts of in vitro L. sibiricus plant extracts, which had the strongest antioxidant properties, may be responsible for stimulating CHO cells to repair oxidatively induced DNA damage, as well as protecting DNA via enhanced activation of the antioxidant genes (SOD2, CAT, and GPx) regulating intracellular antioxidant capacity. The content of phenolic compounds in in vitro raised plants was greater than the levels found in plants propagated from seeds.
益母草在亚洲和欧洲作为传统草药已使用多年。已知该物种具有抗菌、抗炎和抗氧化活性，并已证明可减少细胞内活性氧。所有测试的益母草提取物在暴露于过氧化氢的中国仓鼠卵巢（CHO）细胞中均显示出保护作用和刺激DNA修复的作用。用0.5mg/mL的植物提取物对CHO细胞进行预孵育后，抗氧化基因（SOD2、CAT和GPx）的表达水平升高。液相色谱-串联质谱（LC-MS/MS）和高效液相色谱（HPLC）分析表明，益母草植物提取物中存在9种酚类化合物：儿茶素、毛蕊花糖苷、两种黄酮类化合物（槲皮素和芦丁）以及5种酚酸（4-羟基苯甲酸、绿原酸、咖啡酸、对香豆酸和阿魏酸）。体外培养的益母草植物提取物的根和地上部分具有最强的抗氧化性能，可能是刺激CHO细胞修复氧化诱导的DNA损伤以及通过增强调节细胞内抗氧化能力抗氧化基因（SOD2、CAT和GPx）的激活来保护DNA的原因。体外培养植物中酚类化合物的含量高于种子繁殖植物中的含量。
