Wilson T J, Firth M N, Powell J T, Harrison F L
AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, UK.
Biochem J. 1989 Aug 1;261(3):847-52. doi: 10.1042/bj2610847.
The partial amino acid sequence of the mouse 14 kDa beta-galactoside-binding lectin has been deduced from cDNA clones corresponding to 86% of the coding sequence and extending to the polyadenylation signal. The deduced amino acid sequence for the murine lectin shows 94% identity with the rat, 89% with human, 86% with bovine and 46% with the chicken 14 kDa lectins. A cDNA probe has been used to analyse genomic DNA and identify a single mRNA of approx. 570 bp in 3T3 fibroblasts, murine erythroleukaemia cells and the murine basement-membrane-secreting Engelbreth-Holm-Swarm tumour. Analysis of free and bound polyribosomes has shown that the lectin message is translated on free cytoplasmic ribosomes.
从小鼠14 kDaβ-半乳糖苷结合凝集素的cDNA克隆中推导得到了部分氨基酸序列，该克隆对应86%的编码序列并延伸至聚腺苷酸化信号。推导得到的小鼠凝集素氨基酸序列与大鼠的序列有94%的同一性，与人类的有89%，与牛的有86%，与鸡的14 kDa凝集素有46%的同一性。一个cDNA探针已被用于分析基因组DNA，并在3T3成纤维细胞、小鼠红白血病细胞和分泌小鼠基底膜的恩格尔布雷特-霍尔姆-斯旺肿瘤中鉴定出一个约570 bp的单一mRNA。对游离和结合多核糖体的分析表明，凝集素信息是在游离的细胞质核糖体上翻译的。
