Steinmetz M, Frelinger J G, Fisher D, Hunkapiller T, Pereira D, Weissman S M, Uehara H, Nathenson S, Hood L
Cell. 1981 Apr;24(1):125-34. doi: 10.1016/0092-8674(81)90508-0.
We constructed cDNA libraries from poly(A)+ RNA isolated from cell lines of two different inbred strains of mice, and screened the libraries with a cDNA clone encoding a human transplantation antigen. Three cDNA clones were identified, sequenced and found to encode amino acid sequences highly homologous to portions of a known mouse transplantation antigen. Comparison of the cDNA sequences of mouse transplantation antigens with the constant region domains of the mouse immunoglobulin mu gene reveals a striking homology, which suggests that the two genes share a common ancestor. Antibody genes undergo DNA rearrangement during B cell differentiation that are correlated with their expression. In contrast, DNA blots with these cDNA probes suggest that the genes for the transplantation antigens are not rearranged in the genomes of liver or embryo cells, which express these antigens, as compared with sperm cells, which do not express these antigens. In Bam Hl-digested liver DNAs from different inbred strains of mice, 10-15 bands of hybridization were found. Accordingly, the genes encoding the transplantation antigens appear to constitute a multigene family with similar gene numbers in different mice.
我们从两种不同近交系小鼠的细胞系中分离出的聚腺苷酸加尾（poly(A)+）RNA构建了cDNA文库，并用编码人移植抗原的cDNA克隆筛选这些文库。鉴定出三个cDNA克隆，进行测序后发现它们编码的氨基酸序列与已知小鼠移植抗原的部分高度同源。将小鼠移植抗原的cDNA序列与小鼠免疫球蛋白μ基因的恒定区结构域进行比较，发现了显著的同源性，这表明这两个基因有共同的祖先。抗体基因在B细胞分化过程中会发生与表达相关的DNA重排。相比之下，用这些cDNA探针进行的DNA印迹分析表明，与不表达这些抗原的精子细胞相比，在表达这些抗原的肝脏或胚胎细胞基因组中，移植抗原的基因没有重排。在来自不同近交系小鼠的经Bam Hl酶切的肝脏DNA中，发现了10 - 15条杂交带。因此，编码移植抗原的基因似乎构成了一个多基因家族，在不同小鼠中基因数量相似。
