Malekpour Afshar Reza, Mollaei Hamid Reza, Zandi Bahare, Iranpour Maryam
Research Center for Tropical and Infectious Disease, Kerman University of Medical Sciences, Kerman, Iran. Email:
Asian Pac J Cancer Prev. 2016 Nov 1;17(11):4907-4911. doi: 10.22034/APJCP.2016.17.11.4907.
Glioblastoma multiforme (GBM) is the most aggressive of the gliomas, a collection of tumors arising from glia in the central nervous system. Possible associations between the human cytomegalovirus (HCMV) and the JC virus with GBM are now attracting interest. Our present aim was to investigate the prevalence of the two viruses in Iranian patients from Kerman’s cities in the south of Iran. In addition, the expression rates of pp65, large T antigen and p53 proteins were assessed and their relation with GBM evaluated using reverse transcription real time PCR (rReal Time PCR) . A total of 199 patients with GBM cancer were enrolled, with mean±SD ages of 50.0±19.5 and 50.7±19.6 years for males and females, respectively. The P53 rate was dramatically low suggesting an aetiological role,. Large T antigen expression was found in JC positive samples, while the PP65 antigen was observed in patients positive for CMV and JC . HCMV products and JC virus with oncogenic potential may induce the development of various tumors including glioblastomas. The JC virus produces an early gene product, T-antigen, which has the ability to associate with and functionally inactivate well-studied tumor suppressor proteins including p53 and pRB .
多形性胶质母细胞瘤（GBM）是最具侵袭性的胶质瘤，是一组起源于中枢神经系统胶质细胞的肿瘤。人类巨细胞病毒（HCMV）和JC病毒与GBM之间可能存在的关联目前正引起人们的关注。我们目前的目的是调查伊朗南部克尔曼市患者中这两种病毒的流行情况。此外，评估pp65、大T抗原和p53蛋白的表达率，并使用逆转录实时PCR（rReal Time PCR）评估它们与GBM的关系。总共招募了199例GBM癌症患者，男性和女性的平均年龄±标准差分别为50.0±19.5岁和50.7±19.6岁。P53率极低，提示其病因学作用。在JC阳性样本中发现大T抗原表达，而在CMV和JC阳性患者中观察到PP65抗原。具有致癌潜力的HCMV产物和JC病毒可能诱导包括胶质母细胞瘤在内的各种肿瘤的发生。JC病毒产生一种早期基因产物T抗原，它能够与包括p53和pRB在内的经过充分研究的肿瘤抑制蛋白结合并使其功能失活。
